Schiff Base Copper(II) : Chelate as a Tool for Immobilization of Protein
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概要
- 論文の詳細を見る
In our previous report a new methodology for intermolecular cross-linking or bridging of protein utilizing a spontaneous chelate formation process was proposed. In this paper the reliability of the process as a tool for protein immobilization has been further evaluated. The chromatographic behavior of tryptophan in a column packed with Sepharose coupled with salicylaldehyde residue showed that the α-amino acid was bound tightly to the gel in the presence of copper(II) ion and was eluted by the addition of ethylenediaminetetraacetate (EDTA). It was also proved that subtilisin modified with an α-amino acid residue was immobilized on the column, and this binding was reversed by the addition of EDTA as well.
- 公益社団法人日本薬学会の論文
- 1989-10-25
著者
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金岡 祐一
Faculty of Pharmaceutical Sciences, Hokkaido University
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谷沢 和隆
Faculty of Pharmaceutical Sciences, Hokkaido University
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金岡 裕一
Faculty Of Pharmaceutical Sciences Hokkaido University
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谷沢 和隆
Faculty Of Pharmaceutical Sciences Hokkaido University
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守屋 克裕
Faculty of Pharmaceutical Sciences, Hokkaido University
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守屋 克裕
Faculty Of Pharmaceutical Sciences Hokkaido University
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