Fluorescence Thiol Reagents : Syntheses and Properties of N-Acridinylmaleimides and Their Derivatives

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概要

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• N-(3-Acridinyl) maleimide (3-AM) and N-(9-acridinyl) maleimide (9-AM) were prepared and their reactions with thiol compounds including egg albumin were examined. Both 3-AM (7) and 9-AM (9) react readily with thiol compounds to form strongly fluorescent addition products. Their absorption and fluorescence spectra were measured, and it was found that they are useful fluorescence thiol reagents. Comparative use of the both reagents will afford information on the states of thiols and they serve as a special kind of hydrophobic probe for microenvironment of thiols in macromolecules.

• 公益社団法人日本薬学会の論文
• 1978-02-25

著者

• 高橋 哲夫

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 金岡 祐一

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 伊藤 和彦

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 町田 實

  Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University

• 関根 隆光

  School of Medicine, Juntendo University

• 町田 実

  Formulation Technology Lab. Development & Technology Div. Chugai Pharmaceutical Co. Ltd.

• 町田 實

  Faculty Of Pharmaceutical Sciences Higashi-nippon-gakuen University

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