Comparison of Deacylation Rates of Chymotryptic Catalysis within an Enantiomeric Pair of p-Nitrophenyl Esters

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概要

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• p-Nitrophenyl esters carrying a chiral acyl group were synthesized. These compounds were shown to meet the requirements of chymotrypsin for the specific binding and the acylation. Therefore, the behavior of p-nitrophenyl esters with chymotrypsin is considered to be virtually identical to that of p-amidinophenyl esters with trypsin which were proposed as "inverse substrates" for the enzyme. These esters, derived from each pair of enantiomers, have been successfully used for the analysis of enantiomeric preference of chymotrypsin at the deacylation stage. The chiral requirement of the enzyme active site for the catalytic efficiency was discussed.

• 公益社団法人日本薬学会の論文
• 1991-10-25

著者

• 金岡 祐一

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 山田 浩之

  Hokkaido Institute Of Pharmaceutical Sciences

• 谷沢 和隆

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 金岡 裕一

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 山田 浩之

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 伊藤 邦彦

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 谷沢 和隆

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 伊藤 邦彦

  Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido

• 山田 浩之

  Faculty Of Pharmaceutical Sciences Hokkaido University

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