Preparation of Fluorescence-Labeled and Cross-Linked Subtilisin
スポンサーリンク
概要
- 論文の詳細を見る
Streptomyces subtilisin inhibitor (SSI) is a protein characterized by both its potent inhibitory activity toward subtilisin and its structure, composed of two homologous subunits. It binds two molecules of subtilisin to form a tetrameric complex.Intermolecularly cross-linked subtilisin is expected to form a polymeric complex with SSI. This could provide a useful model of protein-protein associatin. Therefore, Preparation of fluorescence-labeled and cross-linked subtilisin was carried out.
- 社団法人日本薬学会の論文
- 1990-02-25
著者
-
金岡 祐一
Faculty of Pharmaceutical Sciences, Hokkaido University
-
谷沢 和隆
Faculty of Pharmaceutical Sciences, Hokkaido University
-
真野 勉
Faculty Of Pharmaceutical Sciences Hokkaido University
-
金岡 裕一
Faculty Of Pharmaceutical Sciences Hokkaido University
-
谷沢 和隆
Faculty Of Pharmaceutical Sciences Hokkaido University
関連論文
- Application of Remote Photocyclization with a Pair System of Phthalimide and Methylthio Groups. A Photochemical Synthesis of Cyclic Peptide Models
- A REMOVABLE FUNCTIONAL GROUP IN A PHOTOCHEMICAL MACROCYCLIC SYNTHESIS : REMOTE PHOTOCYCLIZATION WITH A PAIR SYSTEM OF PHTHALIMIDE AND 1,3-DITHIOLANYL GROUPS
- A SYNTHETIC APPROACH TO MACROLIDE MODELS BY THE REMOTE PHOTOCYCLIZATION WITH A PAIR SYSTEM OF PHTHALIMIDE AND METHYLTHIO GROUPS
- Photocyclization of N-Alkoxyalkylphthalimides with Favored δ-Hydrogen Abstraction : Syntheses of Oxazolo [4,3-α] isoindoles and Oxazolo [4,3-α]-isoindole-1-spiro-1'-cycloalkane Ring Systems
- Kinetic Study on the Mechanism of Inhibition of Trypsin and Trypsin-like Enzymes by p-Guanidinobenzoate Ester
- Fluorescence Labeled and Cross-Linked Subtilisin : Kinetic Characteristics and Binding to Streptomyces Subtilisin Inhibitor
- Preparation of Fluorescence-Labeled and Cross-Linked Subtilisin
- Inverse Substrates. XV. Spectrometric Properties of Fluorescence-labeled Acyl Trypsins
- Inverse Substrates. IX. Amidinophenyl Esters derived from Amino Acids and Peptides : Synthesis and Properties as Trypsin Substrates
- Proteolytic Enzymes. V. Further Application of p-Nitrophenyl p-Amidinobenzoate as a Titrant of Trypsin
- Proteolytic Emzymes. III. trans-4-Aminomethylcyclohexane-1-carboxylic Acid Derivatives as Substrates and an Active Site Titrant of Trypsin
- Proteolytic Enzymes. II. Studies on the Requirement for the Active Center of Trypsin with Amidinobenzoates as Substrates
- New Fluorogenic Substrates for Subtilisin
- A Novel Fluorometric Ultramicro Determination of Serum γ-Glutamyltranspeptidase Activity
- Fluorescence Thiol Reagents : Syntheses and Properties of N-Acridinylmaleimides and Their Derivatives
- Synthesis of a Key Fluorogenic Amide, L-Arginine-4-methylcoumaryl-7-amide (L-Arg-MCA) and Its Derivatives. Fluorescence Assays for Trypsin and Papain
- A Novel Fluorescent Thiol Reagent : Syntheses and Electronic Spectra of N-(7-Dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM-3) and the Related Compounds
- A New Fluorogenic Substrate for Aminopeptidase
- N-(3-Fluoranthyl) maleimide (FAM) : A Medium Lifetime Fluorescent Thiol Reagent
- Comparison of Deacylation Rates of Chymotryptic Catalysis within an Enantiomeric Pair of p-Nitrophenyl Esters
- Pyranine Phosphate as a New Fluorogenic Substrate for Acidic and Alkaline Phosphatases
- Characterization of Saccharide Moiety in the Electroplax Sodium Channel
- PHOTOREACTION OF N-2-ALKENYLTHIOPHTHALIMIDES : 1,4-DITHIANE FORMATION BY INTRA- AND INTERMOLECULAR TWO-FOLD PHOTOREACTIONS AND THE X-RAY ANALYSIS OF THE DITHIANES
- Photoreactions of Succinimides with an N-Acyl Group in the Side Chain. Synthesis and Stereochemistry of Tricyclic Pyrrolo[1,2-α]pyrazine Ring Systems
- INTRAMOLECULAR PHOTOCYCLOADDITION OF 3-SUBSTITUTED DITHIOSUCCINIMIDES : FACILE SYNTHESIS AND THE CRYSTAL STRUCTURE OF HIGHLY STRAINED MULTICYCLIC FUSED THIETANE SYSTEM
- Photodecarboxylation of N-Phthaloyl-α-amino Acids
- Photochemistry of the Phthalimide System. I. Photodecarboxylation of N-Phthaloyl-α-amino Acids
- Photoaffinity Labeling of the Electroplax Sodium Channel with Tetrodotoxin Derivatives. II. : Comparison of the Photoreactivity of Different Photoactivable Group in the Tetrodotoxin Binding Site
- Synthesis of μ-Conotoxin GIIIA : A Chemical Probe for Sodium Channels
- Photoaffinity Labeling of a δ-Receptor Component of NG 108-15 Cells with a New Enkephalin Analog(Biological)
- THE PHOTOAFFINITY LABELING WITH A TETRODOTOXIN DERIVATIVE OF A SODIUM CHANNEL PROTEIN FROM THE ELECTRIC EEL
- Photoreactions of Benzoylaminopyridines
- Photorearrangement of N, N-Diacetyl Derivatives of Aniline and Naphthylamines
- Hydrogen Fluoride as an Agent in Organic Reactions. I. Degradation of Polynucleotides with Liquid Hydrogen Fluoride
- The Norrish Type II Reaction of Thiophthalimides Having a Benzylic Hydrogen in an N-Alkyl Side Chain
- Introduction of γ-Glutamyl Residue into Chymotrypsin and Agarose Gel : Application to Cross-Linking and Immobilization of Protein
- Dansylation of Streptomyces Subtilisin Inhibitor : Spectrometric Analysis of the Fluorescence-Labeled Inhibitor
- Schiff Base Copper(II) : Chelate as a Tool for Immobilization of Protein
- Diketene Analogs as β-Lactamase Inhibitor
- Synthesis and Physicochemical Properties of Cyclic Peptides
- Comparative Studies on the Stability of Acyl Enzyme Intermediates : An Approach to the Design of Temporary Inhibitors for Trypsin-like Enzymes
- トリプシン"逆性基質"概念の確立.酵素研究及び新生理活性物質開発への応用
- Predominant Transesterification Reaction Catalyzed by Immobilized Trypsin
- Efficient Tryptic Hydrolysis of Aryl Esters with a Cationic Center in the Leaving Group. Further Characterization of"Inverse Substrates"
- New Aspects of the Aromatic Photosubstitution with Iodopyridines
- Direct Photolysis of Halopyridines in Solutions; Generation of the 2-Pyridyl Cation
- Photo-Arylation. VIII. Photosubstitution Reaction of 2-Fluoropyridine with Indoles
- Photo-Arylation. VII. : Photoreaction of 2-Halopyridine with Indole
- Photo-Arylation. IV. Synthesis of 2-Arylpyridines by Photo-Reaction of 2-Iodopyridine with Substituted Benzenes
- Photochemistry of N-But-3-enyl Thiophthalimides. Intramolecular Thietane Formation
- Bimane Fluorogenic Substrates for Microdetermination of Angiotensin Converting Enzyme Level in Serum
- Novel Fluorogenic Substrates for Phosphodiesterase I
- New Fluorogenic Substrates for Microdetermination of Cathepsin C
- Photochemistry of Conjugated Nitrogen-Thiocarbonyl Systems. VII. : Photoaddition of Quinoline-, Isoquinoline- and Phthalazine-Thione Systems to Olefins
- PHOTOREACTIONS OF THIOBARBITURATES WITH OLEFINS; THIETANE FORMATION BY INTERMOLECULAR PHOTOCYCLOADDITION
- Novel Fluorogenic Substrates Containing Bimane System for Microdetermination of Angiotensin I Converting Enzyme
- Photochemistry of Conjugated Nitrogen-Thiocarbonyl Systems. VI. : Photoaddition of Olefins to 2-Thiouracil and 2-Thioquinazolin-2,4-(1H, 3H)-dione
- New Fluorogenic Substrates for Microdetermination of Carboxypeptidase A
- Intermolecular Photoreactions off Phthalimide-Alkene Systems. Regio- and Stereoselective Oxetane Formation from N-Methylphthalimide and N-Acetylindole Derivatives
- New Water-Soluble Fluorogenic Amine. : 7-Aminocoumarin-4-methanesulfonic Acid (ACMS) and Related Substrates for Proteinases
- Intramolecular Photoreactions of Phthalimide-Alkene Systems. Oxetane Formation of N-(ω-Indol-3-ylalkyl)phthalimides
- Efficient Synthetic Method for Ethyl (+)-(2S,3S)-3-[(S)-3-Methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2-oxiranecarboxylate (EST), a New Inhibitor of Cysteine Proteinases(Medicinal Chemistry,Chemical)
- Photochemistry of Conjugated Nitrogen-Carbonyl Systems. III. : Photosensitized Oxygenation of 3-and 6-Substituted 2-Pyridones(Organic,Chemical)
- INTRAMOLECULAR PHOTOCYCLIZATION OF 3-SUBSTITUTED ALICYCLIC DITHIOIMIDES : FACILE SYNTHESIS OF 2-AZABICYCLOALKANES VIA THE NORRISH TYPE II PROCESS
- Studies of Nucleosides and Nucleotides. XLII. Purine Cyclonucleosides. (9). Synthesis of Adenine Cyclonucleosides having 8,2'-and 8,3'-O-Anhydro Linkages
- Photocyclization of ω-Anilinoalkylphthalimides : A Photochemical Macrocyclic Synthesis
- Photochemical Synthesis of 5,10,11,12,12a, 12b-Hexahydro-12b-hydroxyisoindolo [2,1-α] benz [cd] indol-5-one
- Photolysis of the 2-Azabicyclo [4. 1. 0] heptane-3,5-dione System
- Chemical Modification of Proteins. VI. The Rea tion of N-(p-(2-Benzimidazolyl)phenyl)maleimide (BIPM) with the Thiol Groups of Egg Albumin
- Photo-Affinity Labeling. II. Photolysis of Amido Derivatives of Nitrophenyl Azides
- N-(7-Dimethylamino-4-methyl-coumarinyl) maleimides (DACM) : Novel Fluorescent Thiol Reagents
- Amino Acids and Peptides. III. Synthesis of Some Glycine-Oligopeptides by Way of Pentachlorophenyl Esters
- Fluorescence and Structure of Proteins as measured by Incorporation of Fluorophore. II. Synthesis of Maleimide Derivatives as Fluorescence-Labeled Protein-Sulfhydryl Reagents.
- Polyphosphate Esters as a Synthetic Agent. III. Amide and Peptide Syntheses
- Studies on Protein-Sulfhydryl Reagents. I. Synthesis of Benzimidazole Derivatives of Maleimide ; Fluorescent Labeling of Maleimide.
- INTRAMOLECULAR PHOTOCYCLOADDITION OF THIOSUCCINIMIDES WITH ALKENYL N-SIDE CHAINS : SYNTHESIS OF STRAINED MULTICYCLIC SPIRO-THIETANES
- Polyphosphate Ester as a Synthetic Agent. XII. A Convenient Synthesis of Nitriles by the Dehydration of Amides with PPE
- Photolysis of N, N-Dimethylimidazolidinetrione : A Two Nitrogens-Three Carbonyls System
- A Potentially Useful Fluorogenic Amine, 4-Amino-7-nitrobenz-2-oxa-1,3-diazole. An Application as a Substrate for a Microdetermination of Chymotrypsin
- Fluorescence Reagents as an Analytical Tool for Studies of Thio-nucleosides. Their Reactions with Thiouridines
- Photochemistry of the Phthalimide System. XVI. Photocyclization of N-Methylenebisphthalimides
- 3H-Indoles. IV. Photo-and Peroxide-induced Oxygenation of 2-Ethyl-3H-indoles
- Polyphosphate Ester as a Synthetic Agent. XIII. Syntheses of 2-Substituted Benzoxazoles and Benzthiazoles with PPE
- Polyphosphate Ester as a Synthetic Agent. XI. Synthesis of 2-(1'-Aminoalkyl) benzimidazoles from N-Benzoylamino Acids
- Selective Reduction of Peptide-ester Groups in Aqueous Solution
- Polyphosphate Ester as a Synthetic Agent. VII. The Beckmann Rearrangement and Formation of Amidines with PPE
- Polyphosphate Ester as a Synthetic Agent. IV. A Novel Preparation of Aryl Esters
- Polyphosphate Ester as a Synthetic Agent. V. The Fischer Indole Synthesis with Polyphosphate Ester
- Screening Search for Fluorescent Thiol Reagents : Syntheses of N-Naphthylmaleimide Derivatives with a Dimethylamino or a Methoxy Group as an Auxochrome and Their Electronic Spectra
- Photoaddition of Ethers and Olefins to N-Methylphthalimide
- A Synthesis of Dibenzo (a, h) quinolizidine System by way of Immonium Salt Intermediate
- Fluorescence Properties and Reactions of a Novel Fluorescence Thiol Reagent, N-(7-Dimethylamino-4-methyl-3-coumarinyl) maleimide (DACM)
- Heterocycles related to Nucleotides. VI. Photochemistry of Thiopyrimidines : Photoreduction of Thiouracils and Thiouridines with Sodium Borohydride
- Photoinduced Reactions. IV. Photoreduction of Uracil Derivatives in Formic Acid
- Polyphosphate Ester as a Synthetic Agent. VI. The Bischler-Napieralski Reaction of Tryptamine and Tryptophan Derivatives by Means of Polyphosphate Ester
- Hydrolysis of N-Substituted Maleimides : Stability of Fluorescence Thiol Reagents in Aqueous Media
- Photochemical Synthesis of a Pyridopyrrolo [2,1-α] isoindole System by Cyclization of N-Methylpyridylphthalimides
- Oxidation of Pyrroles with Benzoyl Peroxide : Synthesis of Mono- and Dihydroxypyrrole O-Benzoates and Oxidative Cleavage of Pyrrole-2-methanols
- Heterocycles Related to Nucleotides. VII. Reactions of Dimethyluracil and Dimethylthymine with Benzoyl Peroxide
- Polyphosphate Esters as Synthetic Reagent. I. Synthesis of 2-Substituted Benzimidazoles