A Novel Fluorometric Ultramicro Determination of Serum γ-Glutamyltranspeptidase Activity

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概要

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• A simple and highly sensitive rate assay of serum γ-glutamyltranspeptidase activity is described, using a novel fluorogenic substrate, 7-(γ-L-glutamyl)-4-methylcoumarinylamide. The reaction is initiated by adding 20 μl of serum, and fluorescence development in 1 min due to the 7-amino-4-methylcoumarin liberated at 37℃ is followed directly on a recorder. A close correlation was found between activities measured by the fluorometric method and by a conventional method using a chromogenic substrate, γ-L-glutamyl-p-nitronanilide.

• 公益社団法人日本薬学会の論文
• 1981-11-25

著者

• 高橋 哲夫

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 金岡 祐一

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 関根 隆光

  Department of Biochemistry, School of Medicine, Juntendo University

• 板倉 秀子

  Central Laboratory of Clinical Research

• 浪久 利彦

  Department of Medicine, Liver Unit

• 高橋 哲夫

  School of Medicine, Juntendo University

• 中山 仁

  School of Medicine, Juntendo University

• 関根 隆光

  Department Of Biochemistry School Of Medicine Juntendo University

• 浪久 利彦

  Department Of Medicine Liver Unit

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