Multifunctional Cross-Linking Reagents. I. Synthesis and Properties of Novel Photoactivable, Thiol-Directed Fluorescent Reagents

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概要

• 論文の詳細を見る

• Bifunctional photo-activable fluorescent thiol reagents of a new type were synthesized. A maleimide group was bonded to an azidocoumarin group via a methylene chain as a spacer. Reagents of this type react first with a cysteine residue of a protein through the maleimide group, and then form another bond with an amino acid side chain of the protein upon irradiation with light, through a nitrene group formed from the azide. Although the reagent is non-fluorescent, the products are highly fluorescent. The fluorescence characteristics of model compounds of these reagents are also described.

• 公益社団法人日本薬学会の論文
• 1984-10-25

著者

• 中山 仁

  Faculty Of Pharmaceutical Sciences Kumamoto University

• 武野 大策

  順天堂大・医・生化学第一

• 金岡 裕一

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 関根 隆光

  Department of Biochemistry, School of Medicine, Juntendo University

• 上野 隆

  Department Of Biochemistry School Of Medicine Juntendo University

• 小林 昭彦

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 佐藤 英助

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 武野 大策

  Department of Biochemistry, School of Medicine, Juntendo University

• 佐藤 英助

  Faculty Of Pharmaceutical Sciences Hokkaido University:(present Address)department Of Technology Aom

• 関根 隆光

  Department Of Biochemistry School Of Medicine Juntendo University

• 小林 昭彦

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 中山 仁

  Faculty Of Pharmaceutical Sciences Hokkaido University

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