トリプシン"逆性基質"概念の確立.酵素研究及び新生理活性物質開発への応用
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概要
- 論文の詳細を見る
Esters of p-amidinophenol have been demonstrated to undergo efficient and specific tryptic hydrolysis. These esters are characterized by their linkage, i.e., the site specific groups for the enzyme (charged amidinium) is not involved in their carbonyl groups but in the leaving portion. Thus, these esters were named "inverse substrates" with respect to their structure and kinetic properties. It was also found that these esters were hydrolyzed by various proteases which show trypsin-like specificity. A facile procedure for the preparation of acyl-enzymes carrying non specific residue was described and the potential usefulness of the "inverse substrates" concept was proposed.
- 公益社団法人日本薬学会の論文
- 1985-05-25
著者
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谷沢 和隆
Faculty of Pharmaceutical Sciences, Hokkaido University
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谷沢 和隆
Faculty Of Pharmaceutical Sciences Hokkaido University
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谷沢 和隆
北海道大学薬学部
関連論文
- Kinetic Study on the Mechanism of Inhibition of Trypsin and Trypsin-like Enzymes by p-Guanidinobenzoate Ester
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- Preparation of Fluorescence-Labeled and Cross-Linked Subtilisin
- Inverse Substrates. XV. Spectrometric Properties of Fluorescence-labeled Acyl Trypsins
- Inverse Substrates. IX. Amidinophenyl Esters derived from Amino Acids and Peptides : Synthesis and Properties as Trypsin Substrates
- Proteolytic Enzymes. V. Further Application of p-Nitrophenyl p-Amidinobenzoate as a Titrant of Trypsin
- Proteolytic Emzymes. III. trans-4-Aminomethylcyclohexane-1-carboxylic Acid Derivatives as Substrates and an Active Site Titrant of Trypsin
- Proteolytic Enzymes. II. Studies on the Requirement for the Active Center of Trypsin with Amidinobenzoates as Substrates
- New Fluorogenic Substrates for Subtilisin
- Comparison of Deacylation Rates of Chymotryptic Catalysis within an Enantiomeric Pair of p-Nitrophenyl Esters
- Introduction of γ-Glutamyl Residue into Chymotrypsin and Agarose Gel : Application to Cross-Linking and Immobilization of Protein
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- Diketene Analogs as β-Lactamase Inhibitor
- Synthesis and Physicochemical Properties of Cyclic Peptides
- Comparative Studies on the Stability of Acyl Enzyme Intermediates : An Approach to the Design of Temporary Inhibitors for Trypsin-like Enzymes
- トリプシン"逆性基質"概念の確立.酵素研究及び新生理活性物質開発への応用
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