FORMATION OF SUPEROXIDE BY BENZOFURAZANS IN ESCHERICHIA COLI UNDER AEROBIC INCUBATION

概要

論文の詳細を見る
The superoxide (O_2^<-・>) production in Escherichia coli through the action of benzofurazans (BZs) was examined using the cytochrome c (cyt. c) reduction method. Adding BZs to E. coli cell suspensions caused the cyt. c reduction, which was completely inhibited by superoxide dismutase (SOD). The effects of BZs on cyt. c reduction was in the order of benzofurazan (1) ≈ 4,7-dimethylbenzofurazan (2) ≈ 4,7-dibromobenzofurazan (3) < 4-bromo-6-cyanobenzofurazan (4) < 4,7-dicyanobenzofurazan (5). This was correlated with the toxicity of BZs against E. coli growth (1 ≈ 2 ≈ 3 < 4 < 5) and with the redox potentials of BZs (1 ≈ 2 < 3 < 4 < 5). The formation of compound 5 anion radical in the cell suspensions in the absence of dioxygen (O_2), was determined using ESR spectrum. The ESR signal of the anion radical disappeared with the addition of O_2. The BZs effected the O_2^<-・> production in E. coli cells.
社団法人日本薬学会の論文
1991-05-25