The Rabbit as an Animal Model for Warfarin-Induced Hypoprothrombinemia : The Relationship between Prothrombin Time and Prothrombin Complex Activity

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The rabbit model of warfarin-induced hypoprothrombinemia was statistically investigated in terms of the relation between prothrombin time (PT) and prothrombin complex activity (PCA). Among various thromboplastin reagents examined, Simplastin-A was used because of its relatively distinct endpoint in the manual tilt-tube method and convenient coagulation time. The PCA 60%, selected as a measure for assessing the coagulation recovery induced by various oral vitamin K_1 preparations after experimental hypoprothrombinemia in the previous work (Nagata et al., J. Pharm. Pharmacol., 36,527 (1984)), was confirmed to be a reasonable criterion to judge whether the coagulation recovery reaches normal based on a regression analysis of the compiled standard curves of PCA (n=194). The regression equation was given by log PT(s)=1.78-0.428×logPCA (%), r=0.996. The criterion of PCA 20% or less for maximal warfarin-induced hypoprothrombinemia was also justified (13.5±8.5%, n=102). The advantages of the rabbit model include repeated availability of the same animal (at intervals of more than 3 weeks), and the convenient PT difference between PCA 60% and 20% (6-8s).
公益社団法人日本薬学会の論文
1986-02-25