衝撃式酵素反応装置による酵母核酸よりの5'-Nucleotideの製造

概要

論文の詳細を見る
An impact enzyme reactor devised by the authors is very effective reactor for making 5'-nucleotides from yeast RNA with 5'-phosphodiesterase. 5'-Nucleotide was produced by this reactor as follows : Yeast RNA was mixed with 5'-phosphodiesterase prepared from Penicillium citrinum and reacted in an impact enzyme reactor for 20 min; after this produre, the reaction mixture was filtered and conocentrated using an OSMO-3319 type membrane ultrafilter. The 5'-nucleotides obtained were almost all of the monotype, and consisted of 16.5% of 5'-CMP, 26.7% of 5'-AMP, 35.4% of 5'-GMP and 21.4% of 5'-UMP. With this method, about 61% of the enzyme was recovered. A supply of 40% of the enzyme makes possible a successive operation. This fact should prove to be interest to the enzyme industry.
社団法人日本生物工学会の論文
1974-01-25