アスコルビン酸によるファージ不活化に対するフラビンモノヌクレオチドの促進作用の機序

概要

論文の詳細を見る
The potentiating effect of flavin mononucleotide (FMN) on the inactivation of phage Jl by ascorbic acid (AsA) was previously reported. This paper reports the mechanism of the potentiating effect. Bubbling oxygen through the reaction mixture containing phage Jl, AsA and FMN, and the addition of H_2O_2 or transition metal ions to the reaction mixture enhanced the inactivation of phage by combination of AsA and FMN. In contrast, nitrogen bubbling and the addition of reducing agents, chelating agents or general radical scavengers inhibited the inactivation of phage. The results show that oxygen radicals are involved in the inactivation reaction. Experiments with specific radical scaven-gers, catalase or superoxide dismutase showed that hydroxyl radical (OH.) was mainly responsible for the inactivating effect. Spectrophotometric studies show-ed that FMN increased the autoxidation rate of AsA. These results indicate that FMN could enhance the inactivating effect of AsA by increasing the gen-eration of OH. during autoxidation of AsA.
日本ビタミン学会の論文
1987-07-25