アクチノマイシンDのLactobacillus casei-J 1 ファージ系に対する影響

概要

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Actinomycin D (AMD) exhibited a bacteriostatic action on L. casei S-1. Addition of AMD produced an inhibition of the synthesis of RNA, followed by an inhibition of proteinsynthesis. AMD failed to affect the infectivity of free phage J1 (up to 10 μg/ml), the adsorption of phage, or the injection of phage DNA (up to 1.0 μg/ml). AMD (ca. 0.06 μg/ml) completely inhibited the growth of phage J1. An analysis of the action of AMD on phage growth revealed that AMD probably had two effects. (1) If AMD was added prior to the infection, it inhibited the formation of the matured particle, phage DNA, phage protein having serum-blocking power, and phage endolysin. Pulse treatment with AMD and radiobiological analysis showed that AMD blocked an early stage of phage growth. These results suggest that AMD blocks mRNA synthesis by binding the injected phage DNA and prevents the expression of the genetic information of phage DNA. (2) If AMD was added after the eclipse period had elapsed, it produced an immediate, complete inhibition of infectious phage formation. AMD failed to inhibit the synthesis of phage DNA. These results suggest that AMD blocks maturation.
社団法人日本生物工学会の論文