Purification and IgE-Binding Epitopes of a Major Allergen in the Gastropod Turbo cornutus
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概要
- 論文の詳細を見る
The major allergen (Tur c 1) in the muscle of the gastropod, Turbo cornutus, was isolated by Sephacryl S-300,Mono Q HR 5/5 and TSKgel Phenyl-5PW RP column chromatography. ELISA showed Tur c 1 to react strongly with sera from three individuals sensitive to both mollusks and crustaceans. SDS-PAGE showed Tur c 1 to produce a major band corresponding to a molecular mass of 35 kDa under the reduced condition. Its amino acid composition was characterized by the abundance of Glx, followed by Leu, Ala and Lys in decreasing abundance, and the absence of Trp. In addition to these properties, the determined partial amino acid sequence identified Tur c 1 to be a tropomyosin, as in the case of the known mollusk and crustacean allergens. However, the results of competitive ELISA inhibition experiments suggest that Tur c 1 has an IgE-binding epitope in the C-terminal region which is dissimilar to those proposed for Cra g 1 (the oyster Crassostrea gigas allergen) and Pen i 1 (the shrimp Penaeus indicus allergen).
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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Shiomi K
Department Of Food Science And Technology Tokyo University Of Marine Science And Technology
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Kazuo Shiomi
Department Of Food Science And Technology Tokyo University Of Fisheries
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Shiomi Kazuo
東京海洋大学 食品衛生化学研究室
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Shiomi Kazuo
Department Of Food Science And Technology Tokyo University Of Marine Science And Technology
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NAGASHIMA Yuji
Department of Food Science and Technology, Tokyo University of Marine Science and Technology
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Ishikawa M
Tokyo Univ. Fisheries Tokyo Jpn
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Nagashima Y
Department Of Food Science And Technology Tokyo University Of Marine Science And Technology
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Ishida M
Tokyo Univ. Fisheries Tokyo Jpn
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SHIMAKURA Kuniyoshi
Department of Food Science and Technology, Tokyo University of Fisheries
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ISHIKAWA Masaru
Department of Food Science and Technology, Tokyo University of Fisheries
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ISHIDA Masami
Department of Food Science and Technology, Tokyo University of Fisheries
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Ishikawa Masaru
Department Of Food Science And Technology Tokyo University Of Fisheries
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Nagashima Yuji
Deparment Of Food Science And Technology Tokyo University Of Fisheries
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Ishida Masami
Laboratory Of Marine Biochemistry Tokyo University Of Fisheries
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Ishida Masami
Department Of Food Science And Technology Tokyo University Of Fisheries
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Nakao Yoshiki
Nagahama Institute For Biochemical Science Oriental Yeast Co. Ltd.
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Shimakura Kuniyoshi
Department Of Food Science And Technology Faculty Of Marine Science Tokyo University Of Marine Scien
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Ishikawa Masaru
Department Of Biology Faculty Of Science Ehime University
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