Problems with Methods used to Screen Estrogenic Chemicals by Yeast Two-Hybrid Assays

スポンサーリンク

概要

• 論文の詳細を見る

• We developed a yeast two-hybrid assay using a human-type estrogen receptor (hERα) to screen test chemicals reporting weak estrogenic activity at high concentrations. As a result of exposure to several test chemicals, viable yeast cells showed a decrease in number compared with a control. It was shown that this phenomenon was due to fungicidal effects of the test chemicals within the yeast cell or to inhibition of yeast cell proliferation. Therefore, in performing a yeast two-hybrid assay, it is necessary to monitor yeast cell proliferation by methods such as the measurement of the number of viable cells, and it is necessary to screen test chemicals within a concentration range where these chemicals show neither fungicidal effects towards the yeast cell nor inhibition of yeast cell proliferation.

• 公益社団法人日本薬学会の論文
• 2002-02-01

著者

• Nagashima Y

  Department Of Food Science And Technology Tokyo University Of Marine Science And Technology

• Nagao Yukiko

  Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto

• Nakano Shigeru

  Food Safety Research Institute Nissin Food Products Co. Ltd.

• YAMADA Toshihiro

  Food Safety Research Institute, Nissin Food Products Co., Ltd.

• Tanaka Masaharu

  Food Safety Research Institute Nissin Food Products Co. Ltd.

• Nagao Yasuhiro

  Food Safety Research Institute Nissin Food Products Co. Ltd.

• YAMADA Toshihiro

  Central Research Institute, Nissin Food Products Co., Ltd.,

• NOBUHARA Yoichi

  Central Research Institute, Nissin Food Products Co., Ltd.,

• Nobuhara Yoichi

  Central Research Institute Nissin Food Products Co. Ltd

• Nakano Shigeru

  Central Research Institute, Nissin Food Products Co., Ltd.

• Nagao Yasuhiro

  Central Research Institute, Nissin Food Products Co., Ltd.

• Kobayashi Toru

  Central Research Institute, Nissin Food Products Co., Ltd.

• Tanaka Masaharu

  Central Research Institute, Nissin Food Products Co., Ltd.

• Hirano Satoshi

  Central Research Institute, Nissin Food Products Co., Ltd.

• Hirano S

  Central Research Institute Nissin Food Products Co. Ltd.

• Tanaka M

  Food Safety Research Institute Nissin Food Products Co. Ltd.

• Nakao Yoshiki

  Nagahama Institute For Biochemical Science Oriental Yeast Co. Ltd.

• Kobayashi T

  Central Research Institute Nissin Food Products Co. Ltd.

• Yamada Toshihiro

  Food Safety Research Institute Nissin Food Products Co. Ltd.

関連論文

• Effects of Nonylphenol and Triclosan on Production of Plasma Vitellogenin and Testosterone in Male South African Clawed Frogs (Xenopus laevis)(Biochemistry)
• Reactivity of IgE in fish-allergic patients to fish muscle collagen
• Identification of tumor promotion marker genes for predicting tumor promoting potential of chemicals in BALB/c 3T3 cells
• A Genotoxicity test system based on p53R2 gene expression in human cells
• Detection of Walnut Residues in Processed Foods by Polymerase Chain Reaction
• Acute Toxicity Responses of Two Crustaceans, Americamysis bahia and Daphnia magna, to Endocrine Disrupters
• Reactivity of serum immunoglobulin E to bullfrog Rana catesbeiana parvalbumins in fish-allergic patients
• Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin
• Identification of Collagen as a New Fish Allergen
• Two Classes of Allergens, Parvalbumins and Higher Molecular Weight Substances, in Japanese Eel and Bigeye Tuna
• Ani s 1, the major allergen of Anisakis simplex : Purification by affinity chromatography and functional expression in Escherichia coli
• Reduction in the IgE reactivity of Pacific mackerel parvalbumin by mutations at Ca^-binding sites
• Identification of tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding epitopes
• Primary and secondary structures of grammistins, peptide toxins isolated from the skin secretion of the soapfish Pogonoperca punctata
• Immunological Comparison of Shellfish Allergens by Competitive Enzyme-Linked Immunosorbent Assay
• Purification and IgE-Binding Epitopes of a Major Allergen in the Gastropod Turbo cornutus
• Identification of the Oyster Allergen Cra g 2 as Tropomyosin
• Identification of Parvalbumin as an Allergen in Horse Mackerel Muscle
• Screening for Toxins and Protease Inhibitors in Sea Anemones
• Amino Acid Sequence and Biological Activities of Another Kunitz-type Protease Inhibitor isolated from the Sea Anemone Anthopleura aff.xanthogrammica
• Amino Acid Sequences of Kunitz-type Protease Inhibitors from the Sea Anemone Actinia equina
• Isolation and Properties of Allergenic Proteins in the Oyster Crassostrea gigas
• Purification and Properties of a Proteinaceous Toxin Newly Found in the Roe of Lamprey Lampetra japonica
• Isolation and Amino Acid Sequence of a Polypeptide Toxin from the Sea Anemone Radianthus crispus
• Retention and Biotransformation of Arsenic Compounds Administered Intraperitoneally to Carp
• Interaction of grammistins with lipids and their antibacterial activity
• Isolation and Amino Acid Sequences of Polypeptide Toxins in the Caribbean Sea Anemone Condylactis passiflora
• Effects of Disinfectants on Shiga-Like Toxin Converting Phage from Enterohemorrhagic Escherichia coli O157:H7
• Isolation of Bacterial Strains, Which Hydrolyze Glycyrrhizin and Produce Glycyrrhezic Acid, from Soil
• INHIBITION OF MUTAGENICITY BY GLYCYRRHIZA EXTRACT AND GLYCYRRHIZIN
• STUDIES ON THE PLASMIDS HARBOURED IN STREPTOMYCES ROSEOCHROMOGENUS
• Effects of NTE-122, an Acyl-CoA : Cholesterol Acyltransferase Inhibitor, on Cholesterol Esterification and Lipid Secretion From CaCo-2 Cells, and Cholesterol Absorption in Rats
• Effects of NTE-122, a Novel Acyl-CoA:Cholesterol Acyltransferase Inhibitor, on Cholesterol Esterification and High-Density Lipoprotein-Induced Cholesterol Efflux in Macrophages
• Effects of NTE-122, a Novel Acyl-CoA:Cholesterol Acyltransferase Inhibitor, on Cholesterol Esterification and Secretions of Apolipoprotein B-Containing Lipoprotein and Bile Acids in HepG2
• Cholesterol : Lowering Effects of NTE : 122, a Novel Acyl : CoA:Cholesterol Acyltransferase (ACAT) Inhibitor, on Cholesterol Diet : Fed Rats and Rabbits
• O-18 Development of genotoxicity test system based on a p53R2 gene expression in human cells(MUTAGENICITY)(GENERAL SESSION BY ORAL PRESENTATION)(Proceedings of the 31st Annual Meeting)
• Problems with Methods used to Screen Estrogenic Chemicals by Yeast Two-Hybrid Assays
• 食用油脂の品質にかかわる指標としての過酸化物価の再評価
• NTE-122, an Acyl-CoA:Cholesterol Acyltransferase Inhibitor, Prevents the Progression of Atherogenesis in Cholesterol-Fed Rabbits
• Detection of Walnut Residues in Processed Foods by Polymerase Chain Reaction
• Specific Detection by the Polymerase Chain Reaction of Potentially Allergenic Salmonid Fish Residues in Processed Foods

もっと見る 閉じる

スポンサーリンク