Two Kinds of Neutral Serine Proteinases in Salted Muscle of Anchovy, Engraulis japonica
スポンサーリンク
概要
- 論文の詳細を見る
Two kinds of proteinases, type-I and type-II, were purified or partially purified from salted muscle of anchovy, Engraulis japonica. Mol. wts. of type-I and type-II proteinases were estimated to 25,000 and 37,000, respectively, on electrophoretic analysis. Both proteinases strongly hydrolyzed synthetic tri or tetrapeptide substrates specific to trypsin, α-thrombin, and an activated protein C, while they hardly hydrolyzed Arg-MCA and benzoyl Arg-MCA derivatives. The proteinases were inhibited by common trypsin inhibitors. Optimal pH for the proteinase activities were pH 6.8 (type-I) and pH 7.0 to 7.5 (type-II), and the proteinases showed the highest activities at 45℃ (type-I) and 50℃ (type-II). The N-terminaI amino acid sequence of type-I proteinase, ^1I-^2V-^3G-^4G・・・ (29 residues were identified), was significantly similar to sequences of trypsins and tryptases. Based on these findings, both proteinases were presumed to be kinds of tryptases in E. japonica muscle.
- 社団法人日本農芸化学会の論文
- 1995-06-23
著者
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Ishikawa M
Tokyo Univ. Fisheries Tokyo Jpn
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Ishida M
Tokyo Univ. Fisheries Tokyo Jpn
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ISHIKAWA Masaru
Department of Food Science and Technology, Tokyo University of Fisheries
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Ishida Masami
Laboratory Of Marine Biochemistry Tokyo University Of Fisheries
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NAGAYAMA Fumio
Akita Junior College
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Nagayama F
Akita Junior College
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Ishida Masami
Department Of Food Science And Technology Tokyo University Of Fisheries
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Ishida Masami
Laboratory Of Biochemistry Of Marine Resources Tokyo University Of Fisheries
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Sugiyama Nobuko
Laboratory of Biochemistry of Marine Resources, Tokyo University of Fisheries
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Sato Mariko
Laboratory of Biochemistry of Marine Resources, Tokyo University of Fisheries
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Nagayama Fumio
Laboratory of Biochemistry of Marine Resources, Tokyo University of Fisheries
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Sugiyama Nobuko
Laboratory Of Biochemistry Of Marine Resources Tokyo University Of Fisheries
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Sato Mariko
Laboratory Of Biochemistry Of Marine Resources Tokyo University Of Fisheries
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