生体凍結保存における前処理-凍結過程プロセス設計

概要

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The procedure to determine controlling parameters of pre-freezing and freezing processes of biological tissues are discussed. In the pre-freezing process, the concentration of cryoprotectant is raised stepwise to avoid osmotic stress. The rate of increase of the concentration of cryoprotectant can be chosen by considering the relaxation time of membrane permeability of a biological cell. On the other hand, during freezing process, the initial concentration of the cryoprotectant and the cooling rate must be determined to avoid osmotic stress and freeze injury. Optimum parameters can be obtained by estimating the normalized volume of the cell. The maximum possible size of a biological tissue to be cryopreserved can be estimated from the view point of the viability of its cells.
一般社団法人日本機械学会の論文
1998-11-25