Electroporationによる高分子の細胞膜透過促進 : HepG2を用いた高分子の細胞膜透過係数の測定 : 熱工学,内燃機関,動力など

概要

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Vitrification is one of the ideal methods for cryopreserving cells and tissues. Most of the vitrifying solutions of low toxicity and high vitrifying ability are macromolecules, which do not permeate cell membranes. In the present study, enhancement of membrane permeability by electroporation is discussed. Membrane permeability of human hepatomas (HepG2 cells) to several macromolecules (600-70 000 Da) was measured with laser confocal fluorescent microscopy (LCMS). It was found that under the experimental condition, the membrane permeability of HepG2 to the given molecules is aslarge as 10^<-8> m/sec, and the membrane permeability scarcely affected by the strength of electric field.
一般社団法人日本機械学会の論文
2001-08-25