Electroformation法によるGUV生成挙動の観察に基づく生成機序に関する研究(熱工学,内燃機関,動力など)

概要

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Production of Giant Unilamelar Vesicle (GUV) is one of the key technologies to realize the GUV-cell electrofusion for the mass delivery of cryo- and lyo-protective sugars into living cells. Electroformation is one of the promising techniques for rapid uniform production of GUVs. However, the mechanism of electroformation is still unknown. For enhancing the GUV productivity of electroformation, we in investigate the dynamics of vesicles during electroformation with a real-time microscopy. Phosphate lipids (L-α-Phosphatidylcholine; PC) in ultra-purified water was observed by high-speed-imaging fluorescent microscopy or a con focal laser scanning fluorescent microscopy, followed by the image analysis of liposome dynamics. Experimental results showed that; (1) an appropriate vibration of liposomes along the direction of electric field might promote the growth of liposome to GUV. (2) the movement of liposome is largely due to Coulomb force. (3) the amplitude of liposome vibration decreases as the frequency of electric field increases. It is concluded that the charged-sphere model can well explain the liposome dynamics.
社団法人日本機械学会の論文
2007-01-25