5. ミジンコを用いた生体の凍結保存技術への試み(平成3年度第37回凍結及び乾燥研究会研究報告)

概要

論文の詳細を見る
An attempt has been made to preserve living water fleas (daphnia) under a frozen state at a cryogenic temperature. Although the technique of cryopreservation of living cells, tissues or organs having simple structure and with smaller sizes has been established to a certain extent, the cryopreservation of more complex living organs with larger sizes has not yet been achieved successfully. In this study water fleas with 1-3mm in stature are chosen as the subjects because they are possessed of various kinds of tissues and organs. The water fleas in an aqueous solution of glycerol are first cooled at a controlled rate down to -35℃, and then rapidly frozen in a liquid nitrogen of -196℃. If the rate of pre-cooling and the concentration of glycerol solution used as a cryoprotectant are chosen appropriately, the water fleas can be preserved for a long period of time. They can be recovered to life by thawing them at an optimal rate of warming up. A recovery ratio higher than 80% has been attained. However, there still remains a serious problem because the water fleas thus recovered would not live longer than several hours. They die because of injury to certain organs. The technique should be improved furthermore before completion.
低温生物工学会の論文
1991-10-20