生体凍結保存における細胞膜透過係数の測定〔熱工学, 内燃機関, 動力など〕

概要

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The membrane permeability of a biological cell was measured. Obtaining a reliable information on physico-chemical properties is essential to make clear the freezing process and especially, the prefreezing process which is required to avoid osmotic stress and overgrowth of the ice crystal formed in the intracellular solution. The membrane permeability can be described with Kedem-Katchalsky model's parameters (L_p, ω, σ). Parameters can be defined by measuring the volume change of a single cell suspended in a a solution. PBS or glycerol solutions was used as a suspension for measuring L_p and (σ, ω), respectively. HUVEC (Human Vessel's Endothelial Cell) was used as a test specimen. At first, L_p was measured, and then σ and ω were measured. The analytical solutions of the volume change were used to define L_p and ω, and the numerical solution was used to define σ. Quasi-osmotic equilibrium was assumed for introducing an analytical solution to define ω. With this membrane permeability and the diffusion coefficient, and optimized pre-freezing process might be designed.
一般社団法人日本機械学会の論文
1998-06-25