GUV, Jurkat細胞の電気物性測定と有限要素法による電場解析モデルの検証 : GUV-細胞の電気融合による凍結・乾燥保護物質の高効率導入に関する研究(熱工学,内燃機関,動力など)

概要

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Electrofusion of Giant Unilamellar Vesicle (GUV) and living cell is a gentle method to deliver membrane impermeable cryo-/lyo-protective molecules into living cell for cryo-/lyopreservation. In this method, optimal length and strength of electric pulse are required to initiate GUV-cell fusion. Calculating electric field by finite element method (FEM) might be powerful analytical method to predict optimal pulse length and strength of a deformed and adhered GUV-cell. The objective of this study is to clarify whether FEM model of pulsed GUV or cell could be applicable to predict its electric field, especially the change of membrane potential. First, electric properties of GUV and Jurkat were derived from electrorotation spectra with dipole approximation model. Secondly, the electric field and the membrane potential of single GUV or cell after applying a stepwise electric field were calculated by FEM model using the measured electric properties. At the first stage after applying a step electric field, the calculated membrance potential by FEM model shows good agreement with the membrane potential derived from the dipole approximation model. At the time after complete relaxation of electric field, calculated membrane potential of FEM model is lower than that of dipole approximation model. This might be because both models do not take effects of electric double layers on both sides of membrance into account.
社団法人日本機械学会の論文
2008-07-25