生体凍結保存における細胞の耐浸透圧の測定 : viabilityと評価関数としての最小細胞体積分率

概要

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The critical osmotic pressure that biological cells can endure is measured. Optimal parameters of freezing process as well as pre-freezing process are restricted by the critical osmotic pressure which can be translated into the critical normalized volume of the cell. Viability of the cells is measured after the cells suffered from a certain osmotic pressure, and the viability should be estimated from the view point of bio-physico functions demanded. In this study, endothelial cells and smooth muscle cells composing porcine arteriesw are selected as sample cells. The viability of endothelial cell is estimated by dye-exclusion test, and the viability of smooth muscle cell is estimated by measuring contraction-relaxation rate. Optimal parameters of freezing process as well as pre-freezing process can be obtained by estimating the normalized volume of the cell. The critical normalized volume of cells can be estimated from the measured critical osmotic pressure.
一般社団法人日本機械学会の論文
1998-07-25