Involvement of the TGF-β1 Derived from Megakaryocyte in the PEG-rHuMGDF-Induced Myelofibrosis and Bone Formation

スポンサーリンク

概要

• 論文の詳細を見る

• Bone marrow fibrosis and new bone formation were induced by Pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) injection in the rat. We investigated time course changes of megakaryocyte counts, circulating platelet counts, transforming growth factor-β1 (TGF-β1) levels in the bone marrow and those in platelet-poor plasma (PPP) when rats were injected with PEG-rHuMGDF at a dose of 0.1 mg/kg. Additionally, ultrastructural analysis of the circulating platelet and the bone marrow was performed by electron microscope. PEG-rHuMGDF injection daily for 5 days caused a megakaryocyte hyperplasia on days 5-7[after the commencement of the treatment], myelofibrosis on days 7-10, and new bone formation on days 8-15. TGF-β1 levels in the extracellular fluid of the marrow, megakaryocyte numbers, TGF-β1 levels in the PPP, and circulating platelet counts increased by PEG-rHuMGDF injection, and reached to the maximum level on days 7, 7, 8, and 10, respectively. Ultrastructural analysis showed that circulating platelets had no prominent morphological changes in the PEG-rHuMGDF-treated rats on day 8, compared with vehicle-treated rats. Additionally, there were many platelets or fragments of megakaryocyte around mesenchymal cells, and those fragments deposited in the newly formed bone on day 10. These data suggested that myelofibrosis and new bone formation were induced by the increase of TGF-β1 levels derived from bone marrow megakaryocytes.

• 日本毒性病理学会の論文
• 2002-03-25

著者

• Doi Kunio

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

• Doi Kunio

  Department Of Applied Chemistry Nagoya Institute Of Technology

• Kawahara Jun-ichi

  Pharmaceutical Development Lab. Kirin Brewery Co. Ltd.

• Kawahara Jun-ichi

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• Iijima Rieko

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• HARADA Katsuhiko

  Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd.

• IDE Youichi

  Pharmaceutical Development Laboratories, Kirin Brewery Co., Ltd.

• YAMANAKA Eri

  Pharmaceutical Development Laboratories, Kirin Brewery Co., Ltd.

• NAMIKI Yasuko

  Pharmaceutical Development Laboratories, Kirin Brewery Co., Ltd.

• ISHI Hiromi

  Pharmaceutical Development Laboratories, Kirin Brewery Co., Ltd.

• Ide Youichi

  Pharmaceutical Development Laboratories Development Department Pharmaceutical Division Kirin Brewery

• Ide Youichi

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• Ishi Hiromi

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• Yamanaka Eri

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• Namiki Yasuko

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

• Harada Katsuhiko

  Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.

関連論文

• Differences in bone responses to recombinant human granulocyte colony-stimulating factor between mice and rats
• Involvement of Sex, Strain and Age Factors in High Fat Diet-Induced Obesity in C57BL/6J and BALB/cA Mice
• Recovery from pregnancy-induced down-regulation of cytochrome P450 isozymes (CYPs) protein levels in postpartum rat liver(Proceedings of the 32nd Annual Meeting)
• Age-Dependent Susceptibility to MPTP Neurotoxicity in C57BL Mice : A Tyrosine Hydroxylase-Immunohistochemical Evaluation
• The Susceptibility of Pregnant Mice to Encephalomyocarditis (EMC) Virus Infection on Different Days of Gestation
• 1-β-D-Arabinofuranosylcytosine (Ara-C)-induced Apoptosis in the Rat Fetal Tissues and Placenta
• Gene Expression Profiles in the Liver, Placenta and Fetal Liver in Pregnant Rats Treated with T-2 Toxin (TOXICOGENOMICS AND TOXICOPROTEOMICS) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
• DNA Damages and Changes in Proliferating Activity in the Dorsal Epidermis of UVB-Irradiated WBN/ILA-Ht Rats
• T-2 Toxin-Induced Morphological Changes in Pregnant Rats
• Effects of Exogenous Growth Hormone (GH)-Treatment on Carbon Tetrachloride (CCl_4)-Induced Acute Liver Injury in GH-Deficient Mini Rats
• Effects of T-2 Toxin on the Expression of Connexin 32 in the Mouse Liver
• Effects of T-2 Toxin Treatment on CCl_4-Induced Hepatic Necrosis in Mice
• Liver Responses to Repeated Doses of Carbon Tetrachloride in Mini and Wistar Rats
• O11-07 The Effects of Formaldehyde Inhalation on the Responses to Allergen Challenge in Brown Norway and Fischer 344 Rats
• P-48 Regulation of contact hypersensitivity in BALB/c mice repeatedly elicited with picryl-chloride.(Proceedings of the 27th Annual Meeting)
• Immunohistochemical detection of PTHrP and PTH/PTHrP receptor 1 on the odontoblastic reparative process after actinomycin D treatment in rats(Liver, Alimentary system, Proceedings of the 32nd Annual Meeting)
• Teratologic Studies on Rat Perinates and Offspring from Dams Treated with Ethylnitrosourea (ENU)
• Tissue Distribution and Sequence of T-2 Toxin Induced-Apoptosis
• Changes in Location and Number of Tartrate-Resistant Acid Phosphatase (TRAP)-Positive Cells During the Development of Type II Collagen-Induced Arthritis in DBA/1J Mice
• Immunohistochemical Study on Type II Collagen-Induced Arthritis in DBA/1J Mice
• Immunohistochemical Study on Galactosamine-Induced Subacute Hepatitis in Rats of JCL: Wistar-TGN (ARGHGEN) 1Nts Strain (Mini rats)
• D-Galactosamine Induced Hepatocyte Apoptosis is Inhabited in vivo and in Cell Culture by a Calcium Calmodulin Antagonist, Chlorpromazine, and a Calcium Channel Blocker, Verapamil
• Aortic Atheromatous Lesions Developed in APA Hamsters with Streptozotocin induced Diabetes: A New Animal Model for Diabetic Atherosclerosis. 1. Histopathological Studies
• P6-52 Gene expression analysis in mouse primary cultured hepatocytes exposed to streptozotocin(TOXICOGENOMICS)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
• Immunohistochemical Study on the Deposition of Apolipoprotein E in Cerebral and Islet Amyloidoses in Cynomolgus Monkeys (Macaca fascicularis)
• Immunohistochemical detection of anti-oxidative stress enzymes in the dog brain
• Immunohistochemical detection of tau protein in various non-human animal brains
• テオフィリンを反復投与したF344ラットにおける顎下腺の遺伝子発現の変化
• Changes in Salivary Gene Expression in F344 Rats Exposed to Theophylline
• Gene expression changes induced by theophylline in the salivary glands of rats(Proceedings of the 32nd Annual Meeting)
• Nitrofurazone-induced changes in the rat liver(Proceedings of the 32nd Annual Meeting)
• Immunohistochemical Detection of PTHrP and PTH/PTHrP Receptor 1 on the Odontoblastic Reparative Process after Actinomycin D Treatment in Rats
• Morphological Changes in Salivary Glands Induced by Theophylline in Rats
• Endothelial Hypertrophy in Acute Phase of Arteritis Induced by Fenoldopam, a Vasodilator, in Rats
• RESTITUTIVE RESPONSE OF MINI RAT LIVER TO INJURY INDUCED BY A SINGLE ORAL ADMINISTRATION OF THIOACETAMIDE
• Early Ultrastructural Changes in the Dorsal Skin Epidermis of W5istar-Derived Hypotrichotic WBN/ILA-Ht Rats after UVB-Irradiation
• T-2 Toxin-Induced Apoptosis and c-fos mRNA Expression in Con A-Stimulated Mouse Thymocyte Primary Cultures
• 5-Azacytidine-induced Apoptosis in Lymphoid and Hematopoietic Organs of Adult Mice
• Renal Tubulointerstitial Lesions in Mercuric Chloride—Treated F344 Rats
• The Combination of Fixation Using PLP Fixative and Embedding in Paraffin by the AMeX Method is Useful for Immunohistochemical and Enzyme Histochemical Studies of the Lung
• Age-Related Changes in Susceptibility of Mice to Low-Virulent Mouse Hepatitis Virus (MHV-2-CC) Infection
• Electron Microscopic Study of Subcapsular Cell Hyperplasia in the Adrenal Glands of IQI/Jic Mice
• Subcapsular Cell Hyperplasia and Mast Cell Infiltration in the Adrenal Cortex of Mice: Comparative Study in 7 Inbred Strains
• Correlation of Mast Cells with Spindle Cell Hyperplasia in the Adrenal Cortex of IQI/Jic Mice
• Sequence of and Regional Difference in Apoptotic Index in the Mouse Gastrointestinal Mucous Epithelia after T-2 Toxin Inoculation
• Comparative Study on Picryl Chloride (PCL)-Induced Contact Dermatitis in Female IQI/Jic and BALB/c Mice
• The Role of CD4^+ T Cells in Biphasic Hind Limb Paralysis Induced by the D Variant of Encephalomyocarditis Virus (EMC-D) in DBA/2 Mice
• Histological Characteristics of Respiratory System in Brown Norway Rat
• Nitrofurazone at a High Dose Induces Hepatocyte and Adrenal Necrosis in Rats
• Age-Related Histochemical and Ultrastructural Changes in Renal Glomerular Mesangium of APA Hamsters
• Ultrastructure of the Dorsal Skins of Hairless Descendants Derived from Mexican Hairless Dogs
• Dermal Histology of Hairless Rat Derived from Wistar Strain
• EFFECTS OF T-2 TOXIN ON HEPATOCYTE PROLIFERATION IN NORMAL MICE
• Detection of Viral RNA by Electron Microscopic In situ Hybridization (ISH-EM) in The Germinal Epithelium of Mice Infected with Encephalomyocarditis (EMC) Virus
• Variation in Serum Creatine Phosphokinase Activity as Indicated in Two-Phase EMC-D Virus-Induced Myocarditis
• Involvement of Macrophages in the Development of Encephalomyocarditis (EMC) Virus-Induced Diabetes in Mice
• T-2 Toxin and Apoptosis
• Hydrogen Peroxide-Induced Dermatitis in WBN/Kob-Ht Rats
• Development of Apoptosis and Changes in Apoptosis-related Genes Expression in the Mouse Thymus Following T-2 Toxin-inoculation
• T-2 Toxin Induced Skin Lesions in Hipotrichosis (Ht) Rats
• Process of the Development of T-2 Toxin-induced Apoptosis in the Lymphoid Organs of Mice
• Environmental Effect on Eosinophilic Granulomatous Pneumonia (EGP) in Brown Norway Rats
• Glycoconjugate Expression in Follicle-Associated Epithelium (FAE) Covering the Nasal-Associated Lymphoid Tissue (NALT) in Specific Pathogen-Free and Conventional Rats
• DORSAL SKIN LESIONS IN WBN/Kob-Ht RATS TOPICALLY TREATED WITH H_2O_2
• Lectinhistochemistry of Dorsal Skin of Wistar-derived Hypotrichotic WBN/Ila-Ht Rats
• Diurnal Changes in Intraepithelial Lymphocytes (IELs) in the Small Intestine of Mice
• Prevalence of Autoantibody in Cerebrospinal Fluids from Dogs with Various CNS Diseases
• "P-40 The Age-related Difference in Bone Changes in Rats induced by Recombinant Granulocyte Colony-Stimulating Factor
• Gene expression profiling in streptozotocin treated mouse liver using DNA microarray (TOXICOGENOMICS AND TOXICOPROTEOMICS) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
• Silver Selectivity of Novel Monoazapolythioether Derivatives Bearing a Hydrazone Group in the Solvent Extraction
• Highly Selective Copper(II) Extraction with Oligoethylene Glycol Bis(hydrazone)s as Several Compositional Complexes
• Determination of zearalenone and aflatoxins in maize(Others, Proceedings of the 32nd Annual Meeting)
• Contamination of Deoxynivalenol and T-2 toxin in rice(Others, Proceedings of the 32nd Annual Meeting)
• Pharmacokinetic Characteristics of Labeled-Nivalenoland -FusarenonX in Mice.(GENERAL SESSION BY POSTER PRESENTATION)(DRUG METABOLISM)
• Compositional Variation of Ionizable Crown Ether-Alkali Metal Ion Complexes in the Solvent Extraction Using Several Extraction Solvents
• T-2 TOXIN INDUCED APOPTOSIS IN RAT KERATINOCYTE PRIMARY CULTURES(Mycotoxins, Proceedings of the 32nd Annual Meeting)
• The Role of CD4+ T Cells in Biphasic Hind Limb Paralysis Induced by the D Variant of Encephalomyocarditis Virus (EMC-D) in DBA/2 Mice
• Dorsal Skin Reactions to Sunlight and Artificial Ultraviolet Light in Hairless Descendants of Mexican Hairless Dogs
• Involvement of the TGF-β1 Derived from Megakaryocyte in the PEG-rHuMGDF-Induced Myelofibrosis and Bone Formation
• Histochemical, Lectin-Histochemical and Morphometrical Characteristics of Intestinal Goblet Cells of Germfree and Conventional Mice
• Contact Dermatitis Induced by Chemicals for Hair-dyeing in Hairless Dogs
• Spontaneous Comedones on the Skin of Hairless Descendants of Mexican Hairless Dogs
• Current Research and Future Potential of Computer-Aided Diagnosis(CAD)in Radiology : Introduction to Overviews at Five Institutions Around the World
• Morphological and Biochemical Changes During Aging and Photoaging of the Skin of C57BL/6J Mice
• SOLVENT EXTRACTION AND SPECTROPHOTOMETRIC DETERMINATION OF CADMIUM WITH 2,2'-DIPYRIDYL-2-QUINOLYLHYDRAZONE
• The Combination of Fixation Using PLP Fixative and Embedding in Paraffin by the AMeX Method is Useful for Immunohistochemical and Enzyme Histochemical Studies of the Lung
• T-2 Toxin-Induced Apoptosis and c-fos mRNA Expression in Con A-Stimulated Mouse Thymocyte Primary Cultures
• Picryl Chloride-Induced Contact Dermatitis in IQI/Jic Male Mice
• 5-Azacytidine-induced Apoptosis in Lymphoid and Hematopoietic Organs of Adult Mice
• Distribution and Progression of Articular Lesions in Rats Treated with 6-Sulfanilamidoindazole
• Effects of Granulocyte Colony-Stimulating Factor on the Development of Inflammation in Bleomycin-Induced Lung Injury
• PEG-rHuMGDF Causes Osteogenesis by Stimulating Osteoblast Differentiation and Inhibiting Osteoclast Differentiation in Normal Mice
• Morphological and Biochemical Changes During Aging and Photoaging of the Skin of C57BL/6J Mice
• Development of Apoptosis and Changes in Apoptosis-Related Genes Expression in the Thymus of Nivalenol-Treated Mice
• Early Ultrastructural Changes in the Dorsal Skin Epidermis of Wistar-Derived Hypotrichotic WBN/ILA-Ht Rats after UVB-Irradiation
• Renal Tubulointerstitial Lesions in Mercuric Chloride-Treated F344 Rats
• Comparison of Responses of Respiratory System to Cigarette Smoke Inhalation between Brown Norway and Fischer 344 Rats
• Histopathological Evaluation of Toxicity of 6-Sulfanilamidoindazole in Lipopolysaccharide (LPS)-Sensitive and -Resistant Mice
• Hepatic CYP1A Induction in 3-Methylcholanthrene-Treated Transgenic Rats with Insufficient Blood Growth Hormone
• Gene Expression Profiles in the Fetal Mouse Brain after Etoposide (VP-16) Administration

もっと見る 閉じる

スポンサーリンク