Development of Apoptosis and Changes in Apoptosis-related Genes Expression in the Mouse Thymus Following T-2 Toxin-inoculation

概要

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T-2 toxin is a kind of trichothecene mycotoxin produced by species of the genus Fusarium, and it attacks the tissues containing a large number of actively dividing cells such as lymphoid and hematopoietic tissues. The development of apoptosis and the changes in mRNA expressions of apoptosis-related cell-surface antigen (Fas) and genes (p53, bcl-2, c-myc, and c-fos) were investigated in the thymus up to 9 hours after oral inoculation (HAI) with 10 mg/kg body weight of T-2 toxin in female ICR: CD-1 mice. The number of TUNEL-positive lymphocytes clearly increased in the thymus of T-2 toxin-inoculated mice at 6 and 9 HAI. By agarose gel electrophoresis, DNA ladder was clearly detected at 9 HAI. The c-fos mRNA expression increased prior to the progression of apoptotic cell death in the thymus of T-2 toxin-inoculated mice. As to Fas, p53, bcl-2, and c-myc, their mRNA expressions showed no significant changes through the observation period. The results using p53 knockout mice and Fas disfunctional mutant mice also demonstrated that p53 and Fas had no relation to T-2 toxin-induced apoptosis. These findings suggest that c-fos mRNA induction may be associated with T-2 toxin-induced lymphocyte apoptosis.
日本毒性病理学会の論文
1999-03-01