Imaging of Ca^<2+> Release by Caffeine and 9-Methyl-7-bromoeudistomin D and the Associated Activation of Large Conductance Ca^<2+> -Dependent K^+ Channels in Urinary Bladder Smooth Muscle Cells of the Guinea Pig
スポンサーリンク
概要
- 論文の詳細を見る
Ca2+ release by caffeine and 9-methyl-7-bromoeudistomin D (MBED) and the concomitant activation of large conductance Ca2+-dependent K+ (BK) channels were analyzed using confocal Ca2+ imaging and whole cell voltage-clamp methods in guinea pig urinary bladder smooth muscle cells. Puff application of 3 or 10 mM caffeine for several seconds (2 - 5 s) elicited a large increase in intracellular Ca2+ concentration ([Ca2+]i) and induced a phasic outward current at a holding potential of −40 mV. The phasic outward current was the summation of spontaneous transient outward currents (STOCs) due to marked activation of BK channels and was followed by a short cessation of STOCs. Although the increase in superficial [Ca2+]i by caffeine was faster than that in global [Ca2+]i, the peak [Ca2+]i was identical in these areas. Puff application of 100 μM MBED also markedly enhanced STOCs for a few seconds. This response to MBED was not observed when stored Ca2+ was depleted by caffeine. The increase in [Ca2+]i by MBED occurred mainly in superficial areas. Longer application of 100 μM MBED for 2 min did not induce significant global [Ca2+]i increase but decreased the amount of Ca2+ release and cell shortening during the subsequent application of 10 mM caffeine. These results indicate that short application of MBED releases Ca2+ preferentially from superficial storage sites, presumably due to its slow approach to deeper sites. MBED may be a good pharmacological tool to manipulate selectively the superficial Ca2+ stores related to STOCs.
- 社団法人 日本薬理学会の論文
- 2001-04-01
著者
-
IMAIZUMI Yuji
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoy
-
OHIZUMI Yasushi
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku U
-
WATANABE Minoru
Department of Pharmacology, St. Marianna University School of Medicine
-
OHI Yoshiaki
Department of Pharmacology, Faculty of Medicine, University of Toyama
-
Ohizumi Yasushi
Department Of Pharmaceutical Molecular Biology Graduate School Of Pharmaceutical Sciences Tohoku Uni
-
Ishii Yoko
大分医科大学臨床薬理学
-
Ohi Yoshiaki
Department Of Molecular And Cellular Pharmacology Faculty Of Pharmaceutical Sciences Nagoya City Uni
-
Imaizumi Y
Dep. Of Molecular And Cellular Pharmacology Graduate School Of Pharmaceutical Sciences Nagoya City U
-
Imaizumi Yuji
Department Of Chemical Pharmacolgy Faculty Of Pharmaceutical Sciences Nagoya City University
-
Watanabe Minoru
Department Of Molecular And Cellular Pharmacology Nagoya City University
-
ATSUKI Kaoru
Department of Molecular and Cellular Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City U
-
TORII Yuichi
Department of Molecular and Cellular Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City U
-
Ohizumi Yasushi
Department Of Pharmaceutical Molecular Biology Faculty Of Pharmaceutical Sciences Tohoku University
-
Torii Yuichi
Department Of Molecular And Cellular Pharmacology Faculty Of Pharmaceutical Sciences Nagoya City Uni
-
Atsuki Kaoru
Department Of Molecular And Cellular Pharmacology Faculty Of Pharmaceutical Sciences Nagoya City Uni
-
Ishii Yatsuka
Department Of Clinical Pharmacology And Therapeutics Oita Medical University
-
Watanabe Minoru
Department Of Chemical Pharmacolgy Faculty Of Pharmaceutical Sciences Nagoya City University
-
Ohizumi Yasushi
Department Of Anti-dementia Functional Food Development Graduate School Of Engineering Tohoku Univer
-
Watanabe M
Department Of Molecular And Cellular Pharmacology Faculty Of Pharmaceutical Sciences Nagoya City Uni
関連論文
- Shishihakuhito, a traditional Chinese medicine for atopic dermatitis, inhibits IgE-mediated histamine release from rat RBL-2H3 basophilic leukocyte cells
- Regulation of Ryanodine Receptor-Mediated Ca^ Release in Vas Deferens Smooth Muscle Cells
- A NOVEL MODEL OF CONTINUOUS DEPLETION OF GLUTATHIONE IN MICE TREATED WITH L-BUTHIONINE (S,R)-SULFOXIMINE
- 21C-04-2 Correlation between Localization of CYP1A1/2 and Aryl Hydrocarbon Receptor in the Liver Lobule of Rats Treated with 3-methylcholantherene(3MC) or β-Naphthoflavone (BNF).
- Effects of Growth Hormone on Bone in Mini Rats
- Comparison of Bone Changes between Neonatally Monosodium Glutamate-Treated Rats and Mini Rats
- Effects of phenobarbital, a drug-metabolizing enzymes inducer, on hepatic DNA ploidy in hepatectomized rats
- Individual Metabolic Capacity Evaluation of Cytochrome P450 2C19 by Protein and Activity in the Small Intestinal Mucosa of Japanese Pancreatoduodenectomy Patients
- A Novel Action of Palmitoyl-L-carnitine in Human Vascular Endothelial Cells
- α-Mangostin Induces Ca^-ATPase-Dependent Apoptosis via Mitochondrial Pathway in PC12 Cells
- Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes
- Expression of CYP3A4 mRNA Is Correlated With CYP3A4 Protein Level and Metabolic Activity in Human Liver
- Effects of Ovariectomy and Estrogen Replacement on Aota Angiotensin-Converting Enzyme Activity in Rats
- Effect of d-Pseudoephedrine on Cough Reflex and Its Mode of Action in Guinea Pigs
- TRPV4-Like Non-selective Cation Currents in Cultured Aortic Myocytes
- Cell-Culture-Dependent Change of Ca^ Response of Rat Aortic Myocytes to Sphingosine-1-Phosphate
- Docetaxel and carboplatin combination chemotherapy for recurrent endometrial cancer
- Survival of patients with advanced ovarian cancer treated with intermittent chemotherapy following cytoreductive surgery and adjuvant chemotherapy
- Relationship between Patients' Clinical Background and Lithium Concentration in Plasma : An Analysis of TDM Database in a University Hospital in Japan
- Changes in pH Increase Perfusion Pressure of Coronary Arteries in the Rat
- The Mechanism of Rabbit Platelet Aggregation Induced by 2,5-Di-(tert-butyl)-1,4-benzohydroquinone, an Inhibitor of Endoplasmic Reticulum Ca^-ATPase
- The Mode of Inhibitory Action of α-Mangostin, a Novel Inhibitor, on the Sarcoplasmic Reticulum Ca^-Pumping ATPase from Rabbit Skeletal Muscle
- Utilization of Human Liver Microsomes to Explain Individual Differences in Paclitaxel Metabolism by CYP2C8 and CYP3A4
- Osteosarcomas in the Lungs of an ICR Mouse
- Two New Monoterpene Peroxide Glycosides from Aster scaber
- High-performance liquid chromatographic method for determination of DDT and its degradation products in rat plasma, liver and brain : validation and application to a pharmacokinetic study
- The analytical method for determination of DDT and its metabolite in rat plasma, liver and brain, and their concentrations after single oral administration.
- A FURTHER STUDY ON THE RELIABILITY OF TOXICOKINETIC PARAMETERS FOR PREDICTING HEPATOTOXICITY IN RATS RECEIVING A 28-DAY REPEATED ADMINISTRATION OF DDT
- A COMPARATIVE STUDY ON THE RELIABLILITY OF TOXICOKINETIC PARAMETERS FOR PREDICTING HEPATOTOXICITY OF DDT IN RATS RECEIVING A SINGLE OR REPEATED ADMINISTRATION
- Antagonism of Saikosaponin-Induced Prostaglandin E_2 Release by Baicalein in C6 Rat Glioma Cells
- RESPONSES OF ISOLATED RAT IRIS MUSCLES TO SOME AGENTS AND ELECTRICAL STIMULATION
- Nobiletin, a Citrus Flavonoid That Improves Memory Impairment, Rescues Bulbectomy-Induced Cholinergic Neurodegeneration in Mice
- Effects of Sho-saiko-to, San'o-shashin-to and Scutellariae Radix on Intracellular Ca^ Mobilization in C6 Rat Glioma Cells
- SK4 Encodes Intermediate Conductance Ca^-Activated K^+ Channels in Mouse Urinary Bladder Smooth Muscle Cells
- Inhibitions of Histamine Release and Prostaglandin E_2 Synthesis by Mangosteen, a Thai Medicinal Plant
- Nardosinone Enhances Nerve Growth Factor-Induced Neurite Outgrowth in a Mitogen-Activated Protein Kinase- and Protein Kinase C-Dependent Manner in PC12D Cells
- Nerve Growth Factor-Potentiating Compounds from Picrorhizae Rhizoma
- Enhancement of the Nerve Growth Factor-Mediated Neurite Outgrowth from PC12D Cells by Chinese and Paraguayan Medicinal Plants
- The Powerful Stimulatory Action of 6-O-Acetyl-9-methyl-7-bromoeudistomin D on the Contractile Protein System of Rabbit Skeletal Muscle
- Inhibitory Effect of Mao-Bushi-Saishin-to on Prostaglandin E_2 Synthesis in C6 Rat Glioma Cells(Pharmacology)
- Ephedrae herba in Mao-Bushi-Saishin-To Inhibits IgE-Mediated Histamine Release and Increases cAMP Content in RBL-2H3 Cells
- Inhibition of Microtubule Polymerization by SK&F 96365, a Blocker of Receptor-Linked Ca^ Entry
- Structure Activity Relationship and Molecular Modeling Analysis of Nantenine Derivatives in 5-HT_ and α_ Receptor Antagonistic Activities
- Constituents of a Paraguayan Medicinal Plant Verbena littoralis H. B. K.
- The Combination of Rat Mast Cell and Rabbit Aortic Smooth Muscle Is the Simple Bioassay for the Screening of Anti-allergic Ingreditent from Methanolic Extract of Corydalis Tuber(Pharmacognosy)
- Ephedrae herba (Mao) decreased histamine content in RBL-2H3 cells
- Cell-Cycle-Dependent Regulation of Ca^-Activated K^+ Channel in Jurkat T-Lymphocyte
- Clerodane Diterpenoids and Flavonoids with NGF-Potentiating Activity from the Aerial Parts of Baccharis gaudichaudiana
- Endogenous Gibberellins from Young Shoots of Three Bamboo Species
- An Obstructed Venous Aneurysm Presenting as an Abdominal Mass : Report of a Case
- 404 SUBMAXILLARIECTOMY ENHANCED THE INHIBITORY ACTION OF ANDROGEN PRODUCTION IN TESTIS BY KETOCONAZOLE
- 403 INDOMETHACIN INDUCED REDUCTION OF OVARIAN FUNCTION TO HYPOGONADISM
- DISTURBANCE OR RELATIVELY IMPORTANT ACTIONS OF ANTIHYPERTENSIVES, ANTIFUNGAL AGENT AND OPIATE ANTAGONIST TO THE TESTICULAR STEROIDOGENESIS IN RAT
- Prediction of Plasma concentration profiles in feeding study from the data on single oral administration : Application to toxicokinetic study of DDT in F344 male rats.
- A Novel Opener of Large-Conductance Ca^-Activated K^+ (BK) Channel Reduces Ischemic Injury in Rat Cardiac Myocytes by Activating Mitochondrial K_ Channel
- KB-R7943 reveals possible involvement of Na^+-Ca^ exchanger in elevation of intracellular Ca^ in rat carotid arterial myocytes
- Effects of KRN4884, a Novel K^+ Channel Opener, on Ionic Currents in Rabbit Femoral Arterial Myocytes
- Iridoids and Sesquiterpenoids with NGF-Potentiating Activity from the Rhizomes and Roots of Valeriana fauriei
- Application of Physiologically Active Substances Isolated from Natural Resources to Pharmacological Studies
- Malignancy Grade-Dependent Expression of K^+-Channel Subtypes in Human Prostate Cancer
- Characteristics of the ATP-Induced Ca^-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
- Impaired Psychomoter Performance with Topical Ocular and Nasal Ketotifen
- OPHTHALMIC ARTERY FLOW VELOCITY AFTER PHYSICAL EXERCISE IN HEALTHY MEN
- Methyl-β-cyclodextrin Prevents Ca^-Induced Ca^ Release in Smooth Muscle Cells of Mouse Urinary Bladder
- Voltage-Dependent Ca^-Channel Block by Openers of Intermediate and Small Conductance Ca^-Activated K^+ Channels in Urinary Bladder Smooth Muscle Cells
- Mechanisms Underlying the Activation of Large Conductance CA^-Activated K^+ Channels by Nordihydroguaiaretic Acid
- Usefulness and Limitation of DiBAC_4(3), a Voltage-Sensitive Fluorescent Dye, for the Measurement of Membrane Potentials Regulated by Recombinant Large Conductance Ca^-Activated K^+ Channels in HEK293 Cells
- Ca_-Images of Smooth Muscle Cells and Endothelial Cells in One Confocal Plane in Femoral Artery Segments of the Rat
- Imaging of Ca^ Release by Caffeine and 9-Methyl-7-bromoeudistomin D and the Associated Activation of Large Conductance Ca^ -Dependent K^+ Channels in Urinary Bladder Smooth Muscle Cells of the Guinea Pig
- Diverse Expression of Delayed Rectifier K^+ Channel Subtype Transcripts in Several Types of Smooth Muscles of the Rat
- Molecular Analysis of Non-specific Supersensitivity Induced by AF64A in Rat Iris Smooth Muscle
- Protective Effect of Benidipine Against the Abnormal Elictrical Activity in Single Ventricular Myocytes of the Guinea Pig Under Simulated Ischemic Conditions and Reperfusion
- Effects of AF64A on the mRNA Levels of Muscarinic Receptor Subtypes in the Rat Iris Sphincter
- Supersensitivity by AF64A, a Novel Inhibitor of Neuronal Choline-Uptake, of the Rat Iris Sphincter Smooth Muscle
- Effects of Lactate on Intracellular pH and Hypercontracture During Simulated Ischemia and Reperfusion in Cardiac Ventricular Myocytes of the Guinea Pig
- B-67. Study of Vegetable State Patients after Head Injury
- ENHANCEMENT OF ADRENAL ANDROGEN-BIOSYNTHESIS BY THE ADMINISTRATION OF AA560 NAMED ANTIANDROGEN
- Novel Spliced Variants of Large-Conductance Ca2+-Activated K+-Channel β2-Subunit in Human and Rodent Pancreas
- Ca^ Spark as a Regulator of Ion Channel Activity
- Molecular Cloning of m3 Muscarinic Acetylcholine Receptor in Rat Iris
- MOTOR DYSFUNCTION IN SPONTANEOUS THYMOMA RATS, BUFFALO/MNA
- BIOCHEMICAL STUDY ON SPONTANEOUS THYMOMA RATS WITH MOTOR DYSFUNCTION
- Potential of Lansoprazole as a Novel Probe for Cytochrome P450 3A Activity by Measuring Lansoprazole Sulfone in Human Liver Microsomes
- Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes
- Sesquiterpene Esters from the Fruits of Celastrus orbiculatus
- A 51,272-gate-count Dynamic Optically Reconfigurable Gate Array in a standard 0.35μm CMOS Technology
- Novel Spliced Variants of Large-Conductance Ca^-Activated K^+-Channel β_2-Subunit in Human and Rodent Pancreas
- Cellular Ca^ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na^+-Ca^ Exchanger
- Concise paper: Colestimide, an ion-exchange resin agent, may enhance glucose lowering effect of voglibose as their acute effect
- Polymorphism of warfarin related genes (VKORC1 and CYP2C9) analyzed by the smart amplification process method in patients with atrial fibrillation
- Functional and morphological organization of the nucleus tractus solitarius in the fictive cough reflex of guinea pigs
- Electrical Stimulation Prolongs the Survival Days of Leukemic MiceTreated With Methotrexate
- Metronomic scheduling of a cyclic hexapeptide Ra-VII for anti-angiogenesis, tumor vessel maturation and anti-tumor activity
- Proinsulin C-Peptide Stimulates a PKC/IκB/NF-κB Signaling Pathway to Activate COX-2 Gene Transcription in Swiss 3T3 Fibroblasts
- Shishihakuhito, a Kampo Medicine for Atopic Dermatitis, Prevents Lipopolysaccharide-induced Activation of NF-κB-dependent Transcription in C6 Rat Glioma Cells
- Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-κB-Dependent Transcription in C6 Rat Glioma Cells
- Search for Herbal Extracts with the Activity to Stimulate Tyrosine Hydroxylase Gene Transcription in PC12D Cells
- V-1 Enhances PMA-Induced Activation of NF-κB-Dependent Transcription in PC12D cells
- Comparison of the Effects of Omeprazole and Rabeprazole on Ticlopidine Metabolism In Vitro
- Effect of d-Pseudoephedrine on Cough Reflex and Its Mode of Action in Guinea Pigs