Expression of CYP3A4 mRNA Is Correlated With CYP3A4 Protein Level and Metabolic Activity in Human Liver
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概要
- 論文の詳細を見る
A relatively large amount of human liver tissue was required to determine the exact activity of human hepatic CYP3A. Although, the quantity of available human liver tissue samples is limited. We measured levels of CYP3A4 mRNA by RT-PCR with a radiolabeled primer specific for CYP3A4 and compared mRNA expression with CYP3A4 protein level and metabolic activity in liver. The level of CYP3A4 mRNA was correlated with the levels of CYP3A4 protein and activity. Our results suggest that CYP3A4 protein and activity levels can be predicted from CYP3A4 mRNA levels determined by RT-PCR and using a very small amount of liver tissue.
- 社団法人 日本薬理学会の論文
- 2004-04-01
著者
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KUMAI Toshio
Department of Pharmacology, St. Marianna University School of Medicine
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MATSUMOTO Naoki
Department of Pharmacology, St. Marianna University School of Medicine
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KOBAYASHI Shinichi
Department of Pharmacology, St. Marianna University School of Medicine
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Kobayashi Shinichi
Department Of Pharmacology St Marianna University School Of Medicine
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Kobayashi Shinichi
聖マリアンナ医科大学 小児科学教室
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Matsumoto Naoki
Department Of Pharmacology St Marianna University School Of Medicine
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TANAKA Masami
Department of Internal Medicine, Misato Kenwa Hospital
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Matsumoto Naoki
Department Of Cardiology St. Marianna University School Of Medicine
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Tanaka M
Dep. Of Pathology St. Marianna Univ. School Of Medicine
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WATANABE Minoru
Department of Pharmacology, St. Marianna University School of Medicine
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SUZUKI Satoshi
Non-Profit Organization Human & Animal Bridging Research Organization
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SATOH Tetsuo
Non-Profit Organization Human & Animal Bridging Research Organization
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Watanabe Mayumi
Medicinal Safety Research Laboratories Sankyo Co. Ltd.
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Takagi Masayuki
Dep. Of Pathology St. Marianna Univ. School Of Medicine
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Kobayashi Shinichi
Dep. Of Pharmacology St. Marianna Univ. School Of Medicine
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Kobayashi Shinichi
Department Of Pharmacology School Of Medicine Showa University:shionogi Research Laboratories Shiono
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Watanabe M
The Institute Of Environmental Toxicology
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Kumai T
Dep. Of Pharmacogenomics St. Marianna Univ. School Of Medicine
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Kumai Toshio
Department Of Pharmacology St Marianna University School Of Medicine
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Suzuki Satoshi
Res. Laboratories Hab Res. Organization
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Watanabe Minoru
Department Of Molecular And Cellular Pharmacology Nagoya City University
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Watanabe Minoru
Dep. Of Pharmacology St. Marianna Univ. School Of Medicine
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Tanaka Masami
Tanaka Laboratory of Advanced Technology
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Takagi Masayuki
Department Of Pathology St Marianna University School Of Medicine
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Tanaka Masami
Department Of Applied Chemistry College Of Engineering University Of Osaka Prefecture
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Kobayashi Shinichi
Department Of Biomedical Engineering Graduate School Of Medicine University Of Tokyo
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Matsumoto Naoki
Department Of Pharmacology St. Marianna University School Of Medicine
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Satoh Tetsuo
Non-profit Organization Human & Animal Bridging Research Organization
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Watanabe Minoru
Department Of Chemical Pharmacolgy Faculty Of Pharmaceutical Sciences Nagoya City University
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Kumai Toshio
Department of Pharmacogenomics, St. Marianna University School of Medicine
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Matsumoto Naoki
Department of Anesthesia, Gunma Prefectural Cardiovascular Center
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