<I>Lactobacillus bulgaricus</I>の栄養要求とそれにもとずく培地の改良について : 第1報<I>Lb. bulgaricus</I>の生育因子要求性

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Cultures of <I>Lactobacillus bulgaricus</I> often fail to grow on media commonly used for lactic acid bacteria such as standard plate count agar. This is considered dus to deficiencies of some growth factors required by the organisms. To determine the factors deficient in these media, a survay of growth requirements of six cultures of <I>L. bulgaricus</I> in chemically defined media was done.<BR>All of six cultures required “tween 80” and enzymatic digest of casein for their growth. “Tween 80” could be replaced with “tween 40” plus oleic acid, lecithin or cephalin.<BR>Three cultures required pantethine in a medium containing 0.4γ/ml of calcium pantothenate. However, pantethine could be replaced with higher amounts (e.g., 1γ/ml) or calcium pantothenate. When cysteine level in a medium was reduced to 10γ/ml (one-tenth of usual synthetic media) and cystine was omitted, calcium pantothenate in a concentration of 2γ/ml failed to replace pantotheine. This suggests that “activity coefficient” pantethine/pantothenate is a function of cysteine level in a medium.<BR>One culture identified as<I>Thermobacterium jugurt</I>OLRA-JENSEN did not grow in the absence of lactose even after prolonged incubation period.<BR>None required erotic acid or thymidine, and the latter seemed to be inhibitory to some cultures.
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