Streptococcus thermophilusの培地について

概要

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New liquid and solid media for cultivation and for enumeration of Streptococcus thermophilus 510 (an indicator strain for detecting antibiotics in milk) have been developed. The liquid medium contained per liter: Soytone (Difco), 10g; yeast extract (Difco), 5g; lactose, 10g; sodium succinate (Na2C4H4O4•6 H2O), 20g; K2HPO4, 2g; and KH2PO4, 2g (pH 6.6_??_6.8). The composition of the solid medium was the same as above except that it contained powdered agar (15g per liter) and reduced amount (10g per liter) of sodium succinate. Heavy growth (comparable to that in skim milk) was obtained in the liquid medium and large colonies (over 1mm in diameter) developed on the solid medium. Viable counts of litmus milk cultures as measured on this solid medium were the same as, or slightly more than, the direct microscopic clump counts.
社団法人日本農芸化学会の論文