水産動物臓器の有機燐酸化合物に関する研究-IV : プリン,ピリミジンおよびヌクレオシドの分離定量法について

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A method is presented for chromatographic separation and determination of purines, pyrimidines and nucleosides, using Dowex 1-X 2 (200-400 mesh) column with stepwise elution. The column was washed before use with solvent A, and then bases and nucleosides dissolved in solvent A were poured onto the column. After that they were eluted from the resin with the following solvent systems in order: Solvent A: 0.2 N NH4OH-0.02 N HCl-0.01 N Na2B4O7 Solvent B: 0.15 N NH4OH-0.03 N HCl-0.01 N Na2B4O7 Solvent C: 0.05 N NH4OH-0.04 N HCl-0.01 N Na2B4O7 The sequence of elution was nicotinamide + cytosine, uracil, cytidine, adenosine, guanine, hypoxanthine + uridine, adenine, guanosine and inosine (Fig. 5). Since xanthine and hypoxanthine overlap each other and uric acid is labile in the alkaline solvents, they were separated by using a formic acid system on Dowex 1-X8 (Figs. 1 and 2). The improved method can be used for studies of nucleotide catabolism in the tissues.
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