南極産オキアミの蛋白質分解酵素について

概要

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Proteases were extracted from antarctic krill, Euphausia superba, with borate buffer at pH 8.0 They were partially purified by ammonium sulfate fractionation and gel filtration through Sephadex G-100. Further. purification performed with chromatohraphy on DEAE-Sephadex A-50 separated them into two trypsin-like proteases and a protease of unknown specificity. The trypsin-like proteases were characterized by their hydrolysis of TAME as well as the inhibition of the activity in the presence of soy-bean trypsin inhibitor. However, the inhibition of the two tryptic activities by PMSF was less than that in the case of bovine trypsin. All three proteases showed optimal activity at the physiological pH (around 8.0) of krill body fluids. They were stable only in an alkaline medium and digested carp actomyosin. These results suggest that they are responsible for the rapid autolysis of krill body protein which occurs after catching.
日本水産學會の論文