ニジマス肝臓中のヌクレオチドの死後変化 (水産動物臓器の有機燐酸化合物に関する研究-7,8-)

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Living rainbow trouts, Salmo gairdnerii irideus, were decapitated and stored at an ambient temperature of 0°C. After various lengths of time the livers were homogenized in cold 0.6 N perchloric acid, and nucleotides, nucleosides and bases extracted were determined by anion-exchange chromatography. The details of the analytical procedures have been described previously. Results obtained are summarized in Table 1 and Figs. 1-4. ATP and ADP de-creased rapidly with the accumulation of AMP and IMP. Since the dephosphorylation of nucleotide monophosphates is slower than deamination, AMP and IMP levels were high at the beginning, but after 6 hours both nucleotides and inosine decreased with the accumulation of hypoxanthine and xanthine. These changes are characteristic in comparison with those in the doasal and red lateral muscles which show the ac-cumulation of IMP and inosine for a long time. The level of adenosine was low throughout the experimental period. From these results, it may be postulated that the main pathway of the breakdown of purine nucleotides in the liver proceeds via AMP, IMP (adenosine), inosine, hypoxanthine to xanthine. Nicotinamide-adenine dinucleotide was broken down to ADP-ribose and nico-tinamide. The UDP-sugars, which predominate among uracil nucleotides, disap-peared rapidly.
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