魚類筋肉から粗G-アクチンの抽出と重合能力について

概要

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Attempts were made to extract, from acetone-dried muscle powder of fish, a crude G-actin which would retain its characteristic ability to polymerize. The polymerization of G-actin into F-actin was followed by measuring the increase in extract viscosity upon addi-tion of salt. 1) Crude G-actin retaining the ability of polymerization can be extracted from fish muscle powder with a medium containing ATP. Crude G-actin extracted with distilled water seems to lose the ability of polymerization to some extent, and the addition of ATP to the extracts does not effectively restore this ability. 2) In order to obtain G-actin retaining the ability to polymerize, ATP added to the extracting medium is the most effective and ADP, a little less so, while AMP and IMP are ineffective. 3) The degree of polymerization of the crude G-actin was greater with 0.1M KCI, while with 1mM MgCl2, it did not show any tendency to polymerize. The above results suggést that the fundamental polymerization properties of G-actin from fish muscle are essentially the same as those of rabbit G-actin.
日本水産學會の論文