シロサケ幽門垂たんぱく分解酵素前駆体の存在

概要

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The extraction of inactive precursors of proteases from pyloric caeca of chum salmon, Onchorhyncus keta, was examined. Crude extract was obtained by homogenizing the acetone powder of pyloric caeca in phosphate buffer, pH 8.0 (containing 0.5 mg of soybean trypsin inhibitor/ml and 1 mM DFP). Trypsin and chymotrpsin activities were present slightly in the extract. These activities did not increase even after incubation at 10°C and pH 8.0. On the other hand, when the extract containing bovine trypsin as an activator was incubated at 10°C and pH 8.0, trypsin and chymotrypsin activities increased rapidly. Considerable carboxypeptidase A and B activities were originally observed in this extract, but these activities did not change on incubation at 10°C and pH 8.0. On addi-tion of bovine trypsin to the extract, these activities increased rapidly. From these results, it was concluded that trypsin, chymotrypsin and carboxypeptidase A and B of pyloric caeca of chum salmon were present in the gland and in the exocrine juice as inactive precursors or zymogens and that these inactive precursors are extractable by the method described.
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