Calcium accumulating ability of mitochondria from bovine coronary artery. Comparison with aortic mitochondria.
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概要
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The calcium accumulating ability of mitochondria isolated both from bovine coronary artery and aorta was investigated. Coronary artery and aorta were pretreated with 0.1% collagenase. Cytochrome c oxidase activities of mitochondria isolated from coronary artery and aorta showed 25-fold and 19-fold increases, respectively, as compared with those of each homogenate, whereas NADPH-cytochrome c reductase, potassium-phosphatase and Na+-K+ ATPase activities increased less than 2-fold. This suggests that the isolation procedure is capable of obtaining a subcellular fraction highly enriched with mitochondria.Mitochondrial calcium uptake activity of the coronary artery was approximately 250 nmoles Ca2+/mg protein/10min, and was markedly depressed with metabolic inhibitors such as NaN3, ruthenium red and 2, 4-dinitrophenol. Calcium uptake activity of bovine aortic mitochondria showed similar activity and a similar trend in sensitivity to metabolic inhibitors. By contrast, the onset of the calcium binding reaction of the aortic mitochondria was slower and the azide-sensitivity of the mitochondria to magnesium ATPase activity was lower than those for coronary artery mitochondria. The present study has provided a method for isolation of mitochondria with a high capacity of calcium uptake activity, which may prove meaningful for future physiological and pharmacological evaluation of mitochondrial calcium accumulation in vascular smooth muscle.
- International Heart Journal刊行会の論文
著者
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Takeo Satoshi
Department Of Molecular And Cellular Pharmacology Tokyo University Of Pharmacy & Life Science
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Sakanashi Matao
Department Of Pharmacology And Department Of Pediatrics School Of Medicine University Of The Ryukyus
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