Long-Term Supplementation With Eicosapentaenoic Acid Salvages Cardiomyocytes From Hypoxia/Reoxygenation-Induced Injury in Rats Fed With Fish-Oil-Deprived Diet
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概要
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Dietary supplementation of fish oil containing eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA) has been shown to exert protective effects on ischemic/reperfused hearts. We determined whether deprivation of fish oil from the diet paradoxically enhances susceptibility of cardiomyocytes to hypoxia/reoxygenation-induced injury and whether supplementation with either EPA or DHA overcomes such alterations. Rats were fed with fish-oil-rich (FOR) diet, fish-oil-deprived (FOD) diet alone, FOD diet with EPA (1 g/kg/day), or FOD diet with DHA (1 g/kg/day) for 4 weeks. The FOD diet reduced n-3 polyunsaturated fatty acids (PUFAs) and increased n-6 PUFAs such as linoleic (C18:2) and arachidonic acids (C20:4) in myocardial phospholipids. EPA or DHA supplementation increased its incorporation into phospholipid pools. Cardiomyocytes isolated by treatment with collagenase were subjected to 150 min of hypoxia and subsequent reoxygenation for 15 min. In the FOD diet group, the number of surviving rod-shaped cells after hypoxia and reoxygenation was smaller than that of the FOR group. Supplementation with EPA did not affect the number of rod-shaped cells, but attenuated reoxygenation-induced reduction in the number of square-shaped cells. In contrast, DHA supplementation did not afford any protection. The results suggest that deprivation of fish oil from dietary intake enhances the susceptibility of cardiomyocytes to hypoxic injury, and EPA, but not DHA, is capable of salvaging cardiomyocytes from hypoxia/reoxygenation-induced damage.
- 社団法人 日本薬理学会の論文
- 1998-06-01
著者
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TAKEO Satoshi
Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Sciences
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Nasa Yoshihisa
Department Of Molecular And Cellular Pharmacology Tokyo University Of Pharmacy And Life Science
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Sasaki Hideo
Departments Of Neurology Hematology Metabolism Endocrinology And Diabetology Faculty Of Medicine Yam
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Takeo Satoshi
Department Of Molecular And Cellular Pharmacology Tokyo University Of Pharmacy & Life Science
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HAYASHI Jyun-ichi
Department of Gerontrogy, Kyorin Unuiversity School of Medicine
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HAYASHI Michihiko
Department of Pharmacology, Tokyo University of Pharmacy and Life Science
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Sasaki Hideo
Department Of Cardiology Pulmonology And Nephrology Yamagata University School Of Medicine
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Hayashi J
Kyorin Univ. Tokyo Jpn
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Sasaki Hideo
Department Of Pharmacology Tokyo University Of Pharmacy And Life Science
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Hayashi M
Teijin Inst. Bio‐medical Res. Hino Jpn
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Hayashi Jyun-ichi
Departmen Of Gerontology Kyorin University School Of Medicine
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