Antisense-Inhibition of Plasma Membrane Ca^<2+> Pump Induces Apoptosis in Vascular Smooth Muscle Cells
スポンサーリンク
概要
- 論文の詳細を見る
The effect of antisense oligodeoxynucleotides (ODNs) of plasma membrane Ca2+-pumping ATPase (PMCA) on rat aortic vascular smooth muscle cells (VSMCs) in primary culture was examined. More than 80% of the PMCA expressed in cultured VSMCs was the PMCA-1B subtype. Exposed to antisense ODNs against PMCA-1, not only the expression of the PMCA protein but also mRNA of PMCA-1B was diminished in a concentration-dependent manner. Extracellular Na+-independent 45Ca2+ efflux catalyzed via PMCA was inhibited with antisense ODNs. Both the resting and ionomycin- or ATP-stimulated levels of intracellular Ca2+ were increased by antisense ODNs. Furthermore, prolonged treatment with antisense ODNs caused apoptosis in VSMCs. The occurrence of apoptosis was inhibited by FK506, a potent immunosuppressant. These results demonstrate that the PMCA was specifically inhibited by antisense ODNs and suggest that PMCA plays an important role in regulation of intracellular Ca2+ concentrations, especially at the resting condition to prevent an occurrence of apoptosis that may be induced through the activation of calcineurin.
- 2002-10-01
著者
-
Motomura Shigeru
Department Of Pharmacology Hirosaki University
-
Furukawa Ken-ichi
Department Of Molecular Physiology National Cardiovascular Center Research Institute
-
Motomura S
Department Of Pharmacology Hirosaki University School Of Medicine
-
Ohizumi Yasushi
Department Of Pharmaceutical Molecular Biology Faculty Of Pharmaceutical Sciences Tohoku University
-
Shiratori Miwa
Department Of Pharmaceutical Molecular Biology Graduate School Of Pharmaceutical Sciences Tohoku Uni
-
SASAMURA Satoshi
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku U
-
Sasamura Satoshi
Department Of Pharmaceutical Molecular Biology Graduate School Of Pharmaceutical Sciences Tohoku Uni
-
Ohizumi Yasushi
Department Of Anti-dementia Functional Food Development Graduate School Of Engineering Tohoku Univer
-
Motomura Shigeru
Department of Pharmacalogy, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
関連論文
- Shishihakuhito, a traditional Chinese medicine for atopic dermatitis, inhibits IgE-mediated histamine release from rat RBL-2H3 basophilic leukocyte cells
- Adenosine-Induced Dilation of Coronary Resistance Vessels Is Impaired in Rats with Type 2 Diabetes Mellitus: Possible Role of ATP-Sensitive Potassium Channels and Nitric Oxide
- Calcification of Aortic Smooth Muscle Cells Isolated From Spontaneously Hypertensive Rats
- Impairment of Calcium-Activated Potassium Channels in Endothelium-Derived Hyperpolarizing Factor Responses in Spontaneously Hypertensive Rats
- Contribution of Bone Morphogenetic Protein-2 to Aortic Valve Calcification in Aged Rat
- Assessment of the Effects of L- and N-Type Ca^ Channel Blocking Drugs Using Canine Blood-Perfused Papillary Muscle Preparations
- α-Mangostin Induces Ca^-ATPase-Dependent Apoptosis via Mitochondrial Pathway in PC12 Cells
- OJ-277 Impairment of Calcium-Activated Potassium Channels in Endothelium-Derived Hyperpolarizing Factor Response in Coronary Microcirculation of Spontaneously Hypertensive Rat(Coronary Circulation, Basic/Clinical 3 (IHD) : OJ33)(Oral Presentation (Japanes
- A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells
- 0223 ESTIMATION OF NONMECHANICAL O_2 CONSUMPTION IN RABBIT HEART : COMPARISON OF MECHANICAL UNLOADING AND A METHOD USING 2, 3-BUTANEDIONE MONOXIME
- A Functional RNAi Screen for Runx2-Regulated Genes Associated With Ectopic Bone Formation in Human Spinal Ligaments
- Possible Role of Extracellular Nucleotides in Ectopic Ossification of Human Spinal Ligaments
- Current Topics in Pharmacological Research on Bone Metabolism : Molecular Basis of Ectopic Bone Formation Induced by Mechanical Stress
- Current Topics in Pharmacological Research on Bone Metabolism : Preface
- Changes in pH Increase Perfusion Pressure of Coronary Arteries in the Rat
- Role of Corticotropin-Releasing Factor Receptor Type 2βin Urocortin-Induced Vasodilation of Rat Aortas
- Antisense-Inhibition of Plasma Membrane Ca^ Pump Induces Apoptosis in Vascular Smooth Muscle Cells
- Possible Roles of CTGF/Hcs24 in the Initiation and Development of Ossification of the Posterior Longitudinal Ligament
- Localization of 17-kDa Myosin Light Chain Isoforms in Cultured Aortic smooth Muscle Cells
- The Mechanism of Rabbit Platelet Aggregation Induced by 2,5-Di-(tert-butyl)-1,4-benzohydroquinone, an Inhibitor of Endoplasmic Reticulum Ca^-ATPase
- The Mode of Inhibitory Action of α-Mangostin, a Novel Inhibitor, on the Sarcoplasmic Reticulum Ca^-Pumping ATPase from Rabbit Skeletal Muscle
- Two New Monoterpene Peroxide Glycosides from Aster scaber
- Antagonism of Saikosaponin-Induced Prostaglandin E_2 Release by Baicalein in C6 Rat Glioma Cells
- Chemiluminescent Assay for Phosphatidylcholine Hydroperoxide
- Type of ST segment elevation in Brugada Syndrome is related to transmural conduction delay caused by Na^+ channel inactivation
- Rote of Longitudinal Crista Terminalis Conduction in Sustenance of Atrial Flutter : A study in the Canine Atrial Flutter Model
- Electrogram Characteristics Indicative of a Recurrent Conduction Site After Ablation of the Inferior Vena Cava-Tricuspid Annulus Isthmus : A Study in the Canine Blood-Perfused Atrioventricular Preparation
- Nobiletin, a Citrus Flavonoid That Improves Memory Impairment, Rescues Bulbectomy-Induced Cholinergic Neurodegeneration in Mice
- Effects of Sho-saiko-to, San'o-shashin-to and Scutellariae Radix on Intracellular Ca^ Mobilization in C6 Rat Glioma Cells
- Inhibitions of Histamine Release and Prostaglandin E_2 Synthesis by Mangosteen, a Thai Medicinal Plant
- Nardosinone Enhances Nerve Growth Factor-Induced Neurite Outgrowth in a Mitogen-Activated Protein Kinase- and Protein Kinase C-Dependent Manner in PC12D Cells
- Nerve Growth Factor-Potentiating Compounds from Picrorhizae Rhizoma
- Enhancement of the Nerve Growth Factor-Mediated Neurite Outgrowth from PC12D Cells by Chinese and Paraguayan Medicinal Plants
- The Powerful Stimulatory Action of 6-O-Acetyl-9-methyl-7-bromoeudistomin D on the Contractile Protein System of Rabbit Skeletal Muscle
- Inhibitory Effect of Mao-Bushi-Saishin-to on Prostaglandin E_2 Synthesis in C6 Rat Glioma Cells(Pharmacology)
- Ephedrae herba in Mao-Bushi-Saishin-To Inhibits IgE-Mediated Histamine Release and Increases cAMP Content in RBL-2H3 Cells
- Inhibition of Microtubule Polymerization by SK&F 96365, a Blocker of Receptor-Linked Ca^ Entry
- Structure Activity Relationship and Molecular Modeling Analysis of Nantenine Derivatives in 5-HT_ and α_ Receptor Antagonistic Activities
- Constituents of a Paraguayan Medicinal Plant Verbena littoralis H. B. K.
- The Combination of Rat Mast Cell and Rabbit Aortic Smooth Muscle Is the Simple Bioassay for the Screening of Anti-allergic Ingreditent from Methanolic Extract of Corydalis Tuber(Pharmacognosy)
- Ephedrae herba (Mao) decreased histamine content in RBL-2H3 cells
- Clerodane Diterpenoids and Flavonoids with NGF-Potentiating Activity from the Aerial Parts of Baccharis gaudichaudiana
- Iridoids and Sesquiterpenoids with NGF-Potentiating Activity from the Rhizomes and Roots of Valeriana fauriei
- Application of Physiologically Active Substances Isolated from Natural Resources to Pharmacological Studies
- Imaging of Ca^ Release by Caffeine and 9-Methyl-7-bromoeudistomin D and the Associated Activation of Large Conductance Ca^ -Dependent K^+ Channels in Urinary Bladder Smooth Muscle Cells of the Guinea Pig
- Physical and Pharmacological Regulation of Atrial Natriuretic Peptide Release from the Isolated, Blood-Perfused Right Atrium of the Dog
- FRS-028 Amiodarone not only Reverses Electrical Remodeling but Suppresses Matrix Metalloproteinases 2 Activity in Canine Pacing-induced Persistent Atrial Fibrillation Model(Arrhythmia-Basic : Molecular and Genetic : Basis (A) : FRS4)(Featured Research Ses
- Mechanism of the Effect of Amiodarone in Canine Atrial Fibrillation Model
- Role of Heterogeneity of Recovery from Excitation in the Initiation of Ventricular Arrhythmias in Canine Isolated Blood-Perfused Brugada Syndrome Model
- Role of Circulating Catecholamines in Reflex Tachycardia Induced by Ca Antagonists
- Sesquiterpene Esters from the Fruits of Celastrus orbiculatus
- Stimulation of Sarcoplasmic Reticulum Ca^-ATPase by Gingerol Analogues
- -108-PROTEIN KINASE C ACTRVATION OF SARCOLEMMAL Ca^-ATPase ISOLATED FROM BOVINE AORTIC SMOOTH MUSCLE : Cardiovascular Drugs : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells
- Metronomic scheduling of a cyclic hexapeptide Ra-VII for anti-angiogenesis, tumor vessel maturation and anti-tumor activity
- Proinsulin C-Peptide Stimulates a PKC/IκB/NF-κB Signaling Pathway to Activate COX-2 Gene Transcription in Swiss 3T3 Fibroblasts
- Shishihakuhito, a Kampo Medicine for Atopic Dermatitis, Prevents Lipopolysaccharide-induced Activation of NF-κB-dependent Transcription in C6 Rat Glioma Cells
- Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-κB-Dependent Transcription in C6 Rat Glioma Cells
- Search for Herbal Extracts with the Activity to Stimulate Tyrosine Hydroxylase Gene Transcription in PC12D Cells
- V-1 Enhances PMA-Induced Activation of NF-κB-Dependent Transcription in PC12D cells
- Two Distinct Effects of 12S-Hydroxyeicosatetraenoic Acid on Platelet Aggregation by Zooxanthellatoxin-A and Ionomycin
- Maitotoxin-Induced Phosphoinositide Hydrolysis Is Dependent on Extracellular but Not Intracellular Ca^ in Human Astrocytoma Cells
- Lipophilic β-Adrenoceptor Blocking Drugs Have Strong Membrane Stabilizing Actions on Atrioventricular Nodal and Intraventricular Conduction: Comparison with Their β-Adrenoceptor Antagonizing Actions
- IS THE INCREASED K CONDUCTANCE IN CELL MEMBRANE RESPONSIBLE FOR THE INHIBITORY EFFECT OF ACETYLCHOLINE ON ATRIOVENTRICULAR CONDUCTION? : Antiarrhythmic Drugs : IV Auditorium : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION S
- DROMOTROPIC AND CHRONOTROPIC EFFECTS OF CLASS I ANTIARRHYTHMIC AGENTS IN THE CANINE ISOLATED, BLOOD-PERFUSED PAPILLARY MUSCLE PREPARATIONS : Cardiovascular Drugs : 53 Annual Scientific Meeting, Japanese Circulation Society
- Effects of Chinese and Paraguayan Medicinal Plants on the Duration of Immobility of Mice in the Forced Swimming Test
- Excitatory and Inhibitory Effects of Paraguayan Medicinal Plants Equisetum giganteum, Acanthospermum australe, Allophylus edulis and Cordia salicifolia on Contraction of Rabbit Aorta and Guinea-Pig Left Atrium
- Constituents of a Paraguayan Medicinal Plant Verbena littoralis H.B.K.(Natural Medicine Note)
- Inhibitory Action of Paraguayan Medicinal Plants on 5-Lipoxygenase
- Isolation of Acorane-Type Sesquiterpenes from the Paraguayan Medicinal Plant Elinorus latiflorus Nees(Natural Medicine Note)
- Prevalence of reactivation of hepatitis B virus replication in rheumatoid arthritis patients
- Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells
- Contribution of Bone Morphogenetic Protein-2 to Aortic Valve Calcification in Aged Rat
- Inhibitory Effects of Citrus Polymethoxyflavones, Nobiletin and its Analogues, on Acetylcholinesterase Activity
- 9-Methyl-7-bromoeudistomin D, a Caffeine Like Ca^ Releaser, Probably Binds to Cytochromes P450 1A1 and 2B in Liver Microsomes
- The property of active substance from royal jelly in CRE-mediated transcriptional activity
- Genetic Differences in the Osteogenic Differentiation Potency According to the Classification of Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
- Release of atrial natriuretic peptide from the isolated, blood-perfused right atrium of the dog.
- Histamine H2-receptor mediated positive dromotropic effect in the canine atrioventricular node.
- Reconsideration of vascular selectivity of dihydropyridine calcium antagonists: Comparison of cardiovascular profile of mepirodipine, a novel dihydropyridine consisting of a single stereoisomer with (+)-(S)-(S) conformation, with those of nifedipine and n
- The first demonstration of CGRP-immunoreactive fibers in canine hearts: Coronary vasodilator, inotropic and chronotropic effects of CGRP in canine isolated, blood-perfused heart preparations.
- Effects of OPC-18790, a New Positive Inotropic Agent, on Canine Ventricular Arrhythmias.
- Comparison of cardiovascular effects of pirmenol with those of disopyramide in isolated canine heart preparations cross-circulated with a donor dog.
- Comparison of Cardiovascular Effects of a Novel Class Ic Antiarrhythmic Agent, NIK-244, with Those of Flecainide in Isolated Canine Heart Preparations Cross-Circulated with a Donor Dog.
- Utilization of an Isolated, Blood-Perfused Canine Papillary Muscle Preparation as a Model to Assess Efficacy and Adversity of Class I Antiarrhythmic Drugs.
- Upregulation of N-methyl-D-aspartate receptor subunits and c-Fos expressing genes in PC12D cells by nobiletin