Possible Role of Extracellular Nucleotides in Ectopic Ossification of Human Spinal Ligaments
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概要
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To reveal the involvement of extracellular nucleotides in the ossification process in ossification of the posterior longitudinal ligament of the spine (OPLL), the mRNA expression profiles of P2 purinoceptors, mechanical stress-induced ATP release, and ATP-stimulated expression of osteogenic genes were analyzed in ligament cells derived from the spinal ligament of OPLL patients (OPLL cells) and non-OPLL cells derived from the spinal ligaments of cervical spondylotic myelopathy patients as a control. The extracellular ATP concentrations of OPLL cells in static culture were significantly higher than those of non-OPLL cells, and this difference was diminished in the presence of ARL67156, an ecto-nuclease inhibitor. Cyclic stretch markedly increased the extracellular ATP concentrations of both cell types to almost the same level. P2Y1 purinoceptor subtypes were intensively expressed in OPLL cells, but only weakly expressed in non-OPLL cells. Not only ATP addition but also cyclic stretch raised the mRNA levels of alkaline phosphatase and osteopontin in OPLL cells, which were blocked by MRS2179, a selective P2Y1 antagonist. These increases in the expression of osteogenic genes were not observed in non-OPLL cells. These results suggest an important role of P2Y1 and extracellular ATP in the progression of OPLL stimulated by mechanical stress.
- 2008-01-20
著者
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Toh Satoshi
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine
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Motomura Shigeru
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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KANEMARU Kouta
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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SEYA Kazuhiko
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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FURUKAWA Ken-Ichi
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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Toh Satoshi
Department Of Orthopedic Surgery Hirosaki University Graduate School Of Medicine
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Seya Kazuhiko
Department Of Pharmacology Hirosaki University Graduate School Of Medicine
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Seya K
The Second Department Of Surgery Tohoku University School Of Medicine
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Kanemaru Kouta
Department Of Pharmacology Hirosaki University Graduate School Of Medicine
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UEYAMA Kazumasa
Department of Orthopaedic Surgery, Hirosaki University School of Medicine
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Motomura Shigeru
Department Of Pharmacology Hirosaki University
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Furukawa Ken-ichi
Department Of Molecular Physiology National Cardiovascular Center Research Institute
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YOKOYAMA TORU
Department of Orthopaedic Surgery, Hirosaki University School of Medicine
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Yokoyama Toru
Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine
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Yokoyama Toru
Department Of Mechanical Engineering Faculty Of Engineering Yokohama National University
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Furukawa K
Department Of Pharmacology Hirosaki University Graduate School Of Medicine
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Motomura S
Yamanashi Medical Coll. Yamanashi Jpn
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KISHIYA Masaki
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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SAWADA Toshitada
Department of Pharmacology, Hirosaki University Graduate School of Medicine
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Sakou Takashi
Department Of Pharmacology Hirosaki University Graduate School Of Medicine
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Ueyama Kazumasa
Department Of Orthopaedic Surgery Hirosaki Memorial Hospital
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Toh Satoshi
Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine
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Tsuchida S
Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine
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Kishiya Masaki
Department Of Pharmacology Hirosaki University Graduate School Of Medicine
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Yokoyama Toru
Department Of Orthopaedic Surgery Odate Municipal General Hospital
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Yokoyama Toru
Department Of Anesthesiology And Critical Care Medicine Jichi Medical University
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Kishiya Masaki
Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine
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Kanemaru Kouta
Department Of Pharmacalogy Respiratory Medicine And Nephrology Hirosaki University Graduate School O
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Seya Kazuhiko
Department Of Pharmacalogy Respiratory Medicine And Nephrology Hirosaki University Graduate School O
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Motomura Shigeru
Department of Pharmacalogy, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
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Sawada Toshitada
Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine
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