A Large-Scale Gene Expression Screening on Anti-Infl ammatory Effects of Ketamine and Sevofl urane in Rat C6 Glioma Cells

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In the present study, we have conducted cDNA microarray in C6, rat brain glioma cell line, to assessanti-infl ammatory eff ects of ketamine and sevofl urane in the central nervous system. The cultured C6 cells weretreated with ketamine （0-100 μM） and sevofl urane （0 and 0.66 mM）. Total RNA was extracted from the cells andlabeled with fl uorescent dye and then hybridized with microarray slide, containing 1936 genes. Quantitative analysisof each gene expression was confi rmed by real-time polymerase chain reaction （PCR）. Microarray analyses showedthat ketamine downregulated the expression of 4 proinfl ammatory cytokine genes and upregulated that of 2 antiinflammatory cytokine genes. On the other hand, sevofl urane downregulated the expression of 2 proinfl ammatorycytokines but upregulated that of two other proinfl ammatory cytokines. Furthermore, sevofl urane failed to stimulatethe expressions of anti-infl ammatory cytokines. Although patterns of cytokine expression in response to ketamineand sevofl urane were diff erent from each other described above, both anesthetics downregulated a key cytokine,interleukin（ IL）-1β remarkably in microarray analysis, which was confi rmed by real time PCR. These results suggestthat both ketamine and sevofl urane show mainly anti-infl ammatory properties through the inhibition of IL-1β.
弘前大学の論文