INTERACTION OF PROTEOGLYCANS AND α-ELASTIN IN CONSTRUCTION OF EXTRACELLULAR MATRIX OF HUMAN YELLOW LIGAMENT

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概要

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• Human yellow ligament contains abundant proteoglycan (PG) as the matrix factor, and elastic fiber and collagen fiber as the fibrous components, that construct the extracellular matrix. But the interaction between PGs and elastic fiber has not been clarified, therefore we investigated the interaction between PGs and α-elastin from human yellow ligament. One type of large PG and three types of small PGs (decorin, decorin-subtype and biglycan) were purified by ion-exchange chromatography and hydrophobic chromatography, and α-elastin was isolated by alkali treatment from human yellow ligaments taken at the time of operation. The interaction of the PGs with immobilized α-elastin on a sensor was analyzed by surface plasmon resonance. Each of the small PGs had a specific binding to α-elastin, but the large PG had no binding. The binding sites of small PGs were contained in the protein cores rather than in glycosaminoglycan chains. In addition, the differences in the interaction of the small PGs with α-elastin from normal (NYL) and ossified ligaments (OYL) were investigated. The interactions of the small PGs with α-elastin from the OYL were lower than those with α-elastin from the NYL. There were no differences in the amino acid compositions of α-elastin from the NYL and the OYL, but in the OYL, neodesmosine, which is one of the cross-linking amino acids, was significantly less than in the NYL (p<0.05). This finding indicated that there was difference between the OYL and NYL in the interaction of small PGs with α-elastin.

• 日本結合組織学会の論文

著者

• Toh Satoshi

  Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine

• HARATA Seiko

  Department of Orthopedic Surgery, School of Medicine, Hirosaki University

• Toh Satoshi

  Department Of Orthopedic Surgery Hirosaki University Graduate School Of Medicine

• Takagaki Keiichi

  Department of Glycotechnology, Hirosaki University Graduate School of Medicine

• UEYAMA Kazumasa

  Department of Orthopaedic Surgery, Hirosaki University School of Medicine

• Harata Seiko

  Department Of Orthopedic Surgery School Of Medicine Hirosaki University

• Harata Seiko

  Department Of Orthopaedic Surgery Hirosaki University School Of Medicine

• ITABASHI TAITO

  Department of Orthopaedic Surgery, Hirosaki University School of Medicine

• Itabashi Taito

  Department Of Orthopaedic Surgery Hirosaki University School Of Medicine

• ENDO Masahiko

  Department of Biochemistry, School of Medicine, Hirosaki University

• YUKAWA Masahiro

  Department of Communications and Integrated Systems, Tokyo Institute of Technology

• Sakou Takashi

  Department Of Pharmacology Hirosaki University Graduate School Of Medicine

• Ueyama Kazumasa

  Department Of Orthopaedic Surgery Hirosaki Memorial Hospital

• Toh Satoshi

  Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine

• Yukawa Masahiro

  Department Of Orthopaedic Surgery Hirosaki University School Of Medicine

• Tsuchida S

  Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine

• Takagaki K

  Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine

• Takagaki Keiichi

  Department Of Biochemistry And Molecular Biology Pennsylvania State University College Of Medicine

• Takeuchi Kazunari

  Department Of Orthopaedic Surgery Hirosaki University School Of Medicine

• Endo Masahiko

  Departments Of Biochemistry And Orthopaedic Surgery Hirosaki University School Of Medicine

• Takeuchi Kazunari

  Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine

• Itabashi Taito

  Departments Of Biochemistry And Orthopaedic Surgery Hirosaki University School Of Medicine

• Endo Masahiko

  Department Of Biochemistry School Of Medicine Hirosaki University

• Takagaki Keiichi

  Department Of Orthopaedic Surgery Hirosaki University Graduate School Of Medicine

• 原田 征行

  Aomori Prefectural Central Hospital

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