Characterization of .BETA.-D-xyloside-induced glycosaminoglycans and oligosaccharides in cultured human skin fibroblasts.
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概要
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Human skin fibroblasts were incubated in the presence of a fluorogenic xyloside, 4-methylumbelliferyl β-D-xyloside. Three fluorogenic components were isolated and purified from the culture medium by gel permeation high-performance liquid chromatography. Their structures were then characterized by enzymatic digestion, fast-atom-bombardment mass spectrometry, gas-liquid chromatography, and electrophoresis on cellulose acetate membrane. The results showed that one of the components was a mixture of dermatan sulfate (70%) and chondroitin sulfate (30%), bearing the 4-methylumbelliferone at the reducing termini, and having an average molecular weight of 9, 200. The others had the structures galactosyl-galactosyl-xylosyl-4-methylumbelliferone and galactosyl-xylosy1- 4-methylumbelliferone, respectively, representing the linkage region between the glycos-aminoglycan chains and core protein, except that 4-methylumbelliferone replaced the amino acid. Moreover, it was demonstrated that these oligosaccharides were intermediates of glycosaminoglycan synthesis, not depolymerized products.
- 社団法人 日本生化学会の論文
著者
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Kon Atsushi
Department Of Biochemistry Hirosaki University School Of Medicine
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NAKAMURA Toshiya
Department of Biochemistry, Hirosaki University School of Medicine
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Takagaki Keiichi
Department Of Biochemistry And Molecular Biology Pennsylvania State University College Of Medicine
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Tamura Shinri
Department Of Biochemistry Institute For Development Aging And Cancer Tohoku University
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Endo Masahiko
Department Of Biochemistry School Of Medicine Hirosaki University
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