乾燥した植物培養細胞の高温耐性

概要

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Heat tolerance of suspension cultured Pogonatum inflexum and Oryza sativa cells were examined after desiccation treatment. Pogonatum cells were desiccated at 27℃ under 8% relative humidity (RH). Oryza cells were precultured in a medium containing 0.4 M sucrose for 5 days, and then desiccated at 27℃ under 70% RH. Desiccated Pogonatum cells and Oryza cells were exposed to various high temperatures for 1 h. Non-desiccated Pogonatum cells did not survive the heat treatment at 40℃ for 1 h. However, desiccated Pogonatum cells survived the heat treatment at 40℃ for 1 h. Furthermore, desiccated Pogonatum cells were showed a survival rate of approximately 50%, after heat treatment at 90℃ for 1 h. On the other hand, non-desiccated Oryza cells survived the heat treatment at 50℃ for 1 h. However, desiccated Ozyza cells did not survive the heat treatment at 50℃ for 1 h. These results suggested that desiccated Pogonatum cells acquired high temperature tolerance.
2012-10-15