17. コケ植物体ならびに原糸体の乾燥耐性と乾燥後の超低温保存について(平成14年度第48回低温生物工学会研究報告)

概要

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Gametophytic plants of Pogonatum inflexum and Plagiomnium acutum were desiccated and stored in liquid nitrogen. The moss plants desiccated and stored in liquid nitrogen maintained about 40% (Plagiomnium acutum) or 10% (Pogonatum inflexum) of the viability according to the oxygen production test. From leaves of Pogonatum inflexum stored in liquid nitrogen, protonemata newly regenerated, when they were recultured in liquid medium. Also the protonemata of Pogonatum inflexum were desiccated, rehydrated and recultured. In the microscopic observation, survived cells and dead cells were distinguishable from the difference of morphology. These results suggest that moss plant and protonema are suitable experimental material for study the desiccation and freezing tolerance of plant cells.
低温生物工学会の論文
2002-12-28