イネ懸濁培養細胞の乾燥法による保存

概要

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We attempted cryopreserved cultured rice cells by desiccation method. As routinely subcultured rice cell did not survive desiccation, the cells were precultured with 0.4M sucrose for 5 days. Precultured cell were desiccated under controlled relative humidity (RH) and cryopreserved in liquid nitrogen. High survival rate was obtained in the desiccated slowly under 70% RH for 18 h, however the cells desiccated rapidly under 8% RH did not survived both desiccation and cryopreservation. A culture medium for regrowth appeared to be important to enhance the survival of preserved cells. We removed NH_4NO_3 from and added glutamine to MS medium for the higher survival rate when cultured cryopreserved cells. These results suggest that the preculture treatment, slow desiccation under controlled relative humidity and suitable regrowth culture medium were required for the successful preservation.
2011-04-15