14. ウマスギゴケ培養細胞の乾燥保存における含水量ならびに乾燥過程の生存率に及ぼす効果(平成20年度第54回低温生物工学会年会)

概要

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Suspension cultured cells of Polytrichum commune were desiccated at various relative humidities and cryopreserved in liquid nitrogen. The water content of the desiccated cells varied from 0.06 to 0.24 (g H_2O/g dry weight). As the survival rate of the cells that were desiccated at high relative humidity was higher than those desiccated at low relative humidity, it is likely that the final water content was an important factor effecting the survival rate of the desiccated cells. However the cells that were desiccated by the two step method showed a high survival rate even if the final water content was low. Therefore, the effect of the desiccation process can be considered as important for preservation with high survival rate.
2008-12-30