Isolation and Characterization of Bacteria Which Produce α-Xylosidase and Cultural Conditions for Enzyme Production(Biological Chemistry)
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概要
- 論文の詳細を見る
Two strains of Bacillus species capable of producing α-D-xylosidase were isolated from soil samples by a novel method using glucose oxidase. They were facultatively anaerobic, gram-negative, and mesophilic, and formed ellipsodial, central/terminal endospores. The guanine+cytosine content of the DNA were 50.8 mol% for strain No. 208-918-1 and 44.5 mol% for strain No. 693-1. The profiles of physiological and biochemical features of both strains were almost identical and differed from those of the previously described species of Bacillus. It was found that α-D-xylosidase of strain No. 693-1 was a typical inducible enzyme induced by methyl α-D-xyloside and isopropyl α-D-xyloside. The optimum conditions for the production of α-D-xylosidase were found to be a 1:1 ratio of glucose/peptone and an inducer concentration (methyl α-D-xyloside) of 1.0% in the medium. Moreover, the addition of CaCl_2 into the medium enhanced α-D-xylosidase production.
- 社団法人日本農芸化学会の論文
- 1988-12-23
著者
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YASUI Tsuneo
Institute of Applied Biochemistry, University of Tsukuba
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NAKANISHI KOTOYOSHI
Institute of Enology and Viticulture, Yamanashi University
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Yasui Tsuneo
Institute Of Applied Biochemistry The University Of Tsukuba
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Zong Ning
Institute of Applied Biochemistry, University of Tsukuba
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YAMASATO Kazue
Institute of Applied Microbiology, The University of Tokyo
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Nakanishi Kotoyoshi
Institute Of Applied Biochemistry The University Of Tsukuba
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Nakanishi Kotoyoshi
Department Of Biotechnology Faculty Of Engineering Okayania University
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Zong Ning
Institute Of Applied Biochemistry University Of Tsukuba
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Yamasato Kazue
Institute Of Applied Microbiology The University Of Tokyo
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