Liposome Mediated Delivery of Fluorescein Isothiocyanate Dextran into Yeast Protoplasts
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概要
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The potential of liposomes as vehicles for introducing membrane-impermeable substances into yeast protoplasts was studied using a membrane-impermeable water-soluble fluorescent marker, fluorescein isothiocyanate dextran (FITC-dextran). Simple mixing of FITC-dextran encapsulated in liposomes with protoplasts didnt result in the delivery of liposome-encapsulated FITC-dextran into protoplasts. However, when a liposome-protoplast mixture was treated with glycerol after this incubation, the liposome-encapsulated FITC-dextran was delivered into the protoplasts. Three critical factors affected the efficiency of this delivery: the concentration of the glycerol solution; the concentration of calcium ion in the liposome-protoplast incubation mixture; and the timing of the glycerol treatment. Energy metabolism inhibitors blocked this delivery. Under the best conditions about 70% of yeast protoplasts became fluorescent due to the incorporation of FITC-dextran via liposomes.
- 社団法人 日本農芸化学会の論文
著者
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Yasui Tsuneo
Institute Of Applied Biochemistry The University Of Tsukuba
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NAKANISHI Kotoyoshi
Present address: The Institute of Enology and Viticulture, Yamanashi University
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MOCHIZUKI Kazuya
Institute of Applied Biochemistry, University of Tsukuba
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