Transxylosylation Products of Xylobiose by β-Xylosidase-1 from Penicillium wortmanni IFO 7237(Biological Chemistry)

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概要

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• The transxylosylation reaction products of β-xylosidase-1, excreted by Penicillium wortmanni IFO 7237 using β-(1→4)-xylobiose as substrate, have been separated by chromatography on activated charcoal into four fractions, designated as P-1, P-2, P-3, and P-4, respectively. They were further purified by preparative paper chromatography. The characterization and structural analysis were done by measurement of the degree of polymerization (DP) and specific rotation followed by methylation analysis. Moreover, the enzymatic structural analysis of transxylosylation products, with high performance liquid chromatography (HPLC), allowed the confirmation of each structure. The first product, P-1, was β-(1→3)-xylobiose and the second, P-2, was β-(1→4)-xylotriose, but, P-3 was O-β-D-xylopyranosyl-(1→3)-O-β-D-xylopyranosyl-(1→4)-D-xylopyranose or isomeric xylotriose and P-4 was assumed to be O-β-D-xylopyranosyl-(1→4)-[O-β-D-xylopyranosyl-(1→3)]-O-β-D-xylopyranosyl-(1→4)-D-xylopyranose.

• 社団法人日本農芸化学会の論文
• 1988-05-23

著者

• YASUI Tsuneo

  Institute of Applied Biochemistry, University of Tsukuba

• Kamiyama Yoshi

  Institute Of Applied Biochemistry University Of Tsukuba

• Kamiyama Yoshi

  Institute Of Applied Biochemistiry University Of Tsukuba

• Yasui Tsuneo

  Institute Of Applied Biochemistry Tsukuba University

• Yasui Tsuneo

  Institute Of Applied Biochemistry The University Of Tsukuba

• Yasui Tsuneo

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address) Seitoku Junior College Of

• MATSUO Masaru

  Institute of Applied Biochemistry, Uriiversity of Tsukuba

• 安井 勉

  Department Of Animal Science Faculty Of Agriculture Hokkaido University

• Win Maung

  Institute Of Applied Biochemistry University Of Tsukuba:(present Office)department Of Botany Rangoon

• Matsuo Masaru

  Institute Of Applied Biochemistry Tsukuba University

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