Purification and Some Properties of Aspergillus pulverulentus β-Xylosidase with Transxylosylation Capacity
スポンサーリンク
概要
- 論文の詳細を見る
Two β-xylosidases [EC 3.2.1.37], β-Xyl I (molecular mass 180 kDa, pI 4.7) and β-Xyl II (molecular mass 190 kDa, pI 3.5), derived from Aspergillus pulverulentus were separated and purified by successive chromatographies and their characterization and transxylosylation were studied. β-Xyl I and β-Xyl II were stable at temperatures up to 50℃ and from pH 1.5 to 6.5 and 2.5 to 7.0,respectively, while their highest activities were in the pH ranges 2.5-3.5 and 4.0-5.0 at 60℃. Although both enzymes were strongly inhibited by N-bromosuccinimide, the inhibitory effect of HgCl_2 was not significant on either. The two enzymes exhibited different resistances against AgNO_3,glucono 1,5-lactone and nojirimycin. They were shown to have broad acceptor specificity in transferring the xylosyl residue of xylooligosaccharides to various alcohol and phenolic compound acceptors. In the presence of 25% or more 2-propanol, the synthesis of the transfer product, 2-propyl β-xyloside, was closely consistent with the theoretical yield.
- 社団法人日本生物工学会の論文
- 1995-01-25
著者
-
Kamiyama Yoshi
Institute Of Applied Biochemistry University Of Tsukuba
-
Kamiyama Yoshi
Institute Of Applied Biochemistiry University Of Tsukuba
-
Yasui Tsuneo
Seitoku Junior College of Nutrition
-
Yasui T
Seitoku Junior Coll. Nutrition Tokyo Jpn
-
SULISTYO JOKO
Institute of Applied Biochemistry, University of Tsukuba
-
Sulistyo Joko
Institute Of Applied Biochemistry University Of Tsukuba
関連論文
- Chemical Modification of Xylanases from Streptomyces sp.
- Comparison of Xylan and Methyl β-Xyloside-Induced Xylanases from Streptomyces sp.
- Induction of Membrane-Bound Xylosidase in a Streptomyces sp.
- A New Assay Method for Xylobiose-hydrolysis Activity of β-Xylosidase and Comparison of Several β-Xylosidases
- Screening for β-Mannosidases with Transmannosidation Capacity
- The Condensation Reaction of Aspergillus niger Crude β-Xylosidase Using Xylose
- Enzymatic Synthesis of Alkyl β-Xylosides from Xylobiose by Application of the Transxylosyl Reaction of Aspergillus niger β-Xylosidase (Biological Chemistry)
- Purification and Some Properties of Aspergillus pulverulentus β-Xylosidase with Transxylosylation Capacity
- Enzymatic Synthesis of Hydroquinone β-Xyloside from Xylooligosaccharides
- Transxylosylation Products of Xylobiose by β-Xylosidase-1 from Penicillium wortmanni IFO 7237(Biological Chemistry)
- A Transxylosylation Product, α-D-Xylosylisoprimeverose, Synthesized by Bacillus α-D-Xylosidase from Isoprimeverose(Biological Chemistry)
- Substrate Specificity of Bacillus α-D-Xylosidase(Biological Chemistry)
- Substrate Specificity of α-L-Arabinofuranosidase from Streptomyces diastatochromogenes 065 toward Arabinose-Containing Oligosaccharides
- Synthesis of Alkyl β-Mannosides from Mannobiose by Aspergillus niger β-Mannosidase