Purification and Some Properties of β-1,3-Xylanase from Aspergillus terreus A-07(Biological Chemistry)

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概要

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• A β-1,3-xylan was prepared by sodium hydroxide extraction from seaweeds of Bryopsis maxima and Caulerpa sp. The seaweed xylan obtained contains xylose residues only and β-1,3-linkage was characterized by the method of ^<13>C-NMR spectroscopy and methylation analysis. A strain of Aspergillus terreus A-07 isolated from soil produced an endo-β-1,3-xylanase, decomposing β-1,3-xylan to D-xylose and D-xylooligosaccharides. Six different xylanases in the culture filtrate of A. terreus A-07 have been found and purified to homogeneity from 40- to 95-fold by ammonium sulfate fractionation, SP-Sephadex C-25 ion exchange chromatography, Biogel P-100 gel filtration, and isoelectric focusing. Each of the purified enzymes gave a single band on polyacrylamide disc gel electrophoresis, indicating that all of the purified β-1,3-xylanases were electrophoretically homogeneous. The six kinds of purified enzymes on SDS polyacrylamide gel electrophoresis indicated that two of the β-1,3-xylanases have molecular weights of 11,000, another two of the β-1,3-xylanases have molecular weights of 14,500, and the last two β-1,3-xylanases have molecular weights of 13,500 and 20,000. The pH optima of the β-1,3-xylanases were from 4.0 to 5.5. The optimum temperature for activity was from 40 to 55℃. All of the enzyme activities of the six β-1,3-xylanases were inhibited by Hg^<2+>, Mn^<2+>, and N-bromosuccinimide. With respect to the hydrolysis patterns, the purified β-1,3-xylanases hydrolyzed β-1,3-xylan to xylose and xylooligosaccharides. The β-1,3-xylanases hydrolyzed β-1,3-xylan and rhodymenan but did not hydrolyze β-1,4-xylan, cellulose powder, laminaran, β-1,3-xylobiose or β-1,3-xylotriose. By their action patterns and substrate specificities, all of the six purified enzymes were endo-type β-1,3-xylanases.

• 社団法人日本農芸化学会の論文
• 1986-05-23

著者

• Yasui Tsuneo

  Institute Of Applied Biochemistry Tsukuba University

• Yasui Tsuneo

  Institute Of Applied Biochemistry The University Of Tsukuba

• 安井 勉

  Department Of Animal Science Faculty Of Agriculture Hokkaido University

• Chen Wen

  Institute Of Applied Biochemistry The University Of Tsukuba

• Matsuo Masaru

  Institute Of Applied Biochemistry Tsukuba University

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