Inhibitory Effects of Fidarestat on Aldose Reductase and Aldehyde Reductase Activity Evaluated by a New Method Using HPLC with Post-column Spectrophotometric Detection
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概要
- 論文の詳細を見る
A new method to assay the activity of aldose reductase (AR) and aldehyde reductase (AHR) by high-performance liquid chromatography is described. The separation of AR and AHR from tissue extracts using an anionexchange column was followed by chromatographic measurement of the activity in the elute. AR and AHR activity were expressed as the area under the peak obtained by post-coumn spectrophotometric detection of the decrease of coenzyme (NADPH) in each enzyme reaction. In the enzyme preparation from rat or human tissues obtained by this method, two active peaks were identified as AR and AHR. The conrrelation coefficient between the injection volume of the enzyme preparation from each tissue and each peak area was 0.998 or greater. In addition, the within-day preservation rate of AR or AHR activity from each tissue was over 95%.In a comparative study of fidarestat with other AR inhibitors using this method, it was confirmed that the inhibitory effect of fidarestat on AR activity from each rat tissue was more potent than that produced by sorbinil and equipotent to that of epalrestat and zenarestat. Fidarestat was also found to inhibit AR activity more potently than AHR activity in human erythrocytes. Therefore, this method is applicable to studies of the selective inhibition of AR or AHR by test compounds.
- 公益社団法人日本薬学会の論文
- 2000-02-01
著者
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鈴木 徳治
千葉大 薬
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Tanaka Tomoko
Kobe Pharmaceutical University
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Tanaka T
School Of Pharmaceutical Sciences Nagasaki University
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Tanaka T
Kobe Pharmaceutical University
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MIZUNO Kuniharu
Research and Development Division, Sanwa Kagaku Kenkyusho Co., Ltd.,
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SUZUKI Takeshi
Research and Development Division, Sanwa Kagaku Kenkyusho Co., Ltd.,
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TANAKA Tomoko
Research and Development Division, Sanwa Kagaku Kenkyusho Co., Ltd.,
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TANIKO Kaori
Research and Development Division, Sanwa Kagaku Kenkyusho Co., Ltd.,
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SUZUKI Tsunemasa
Research and Development Division, Sanwa Kagaku Kenkyusho Co., Ltd.,
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Taniko Kaori
Research And Development Division Sanwa Kagaku Kenkyusho Co. Ltd.
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Mizuno Kuniharu
Research And Development Division Sanwa Kagaku Kenkyusho Co. Ltd.
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Suzuki T
Research Unit Of Pharmaceutics College Of Pharmacy Nihon University
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Suzuki Takeshi
Research And Development Division Sanwa Kagaku Kenkyusho Co. Ltd.
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Suzuki Takeshi
Research And Analysis Department Nhk Spring Co. Ltd.
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